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dc.contributor.authorSchede, Halima H
dc.contributor.authorNatarajan, Pradeep
dc.contributor.authorChakraborty, Arup K
dc.contributor.authorShrinivas, Krishna
dc.date.accessioned2024-12-09T21:06:45Z
dc.date.available2024-12-09T21:06:45Z
dc.date.issued2023
dc.identifier.urihttps://hdl.handle.net/1721.1/157802
dc.description.abstractCondensation by phase separation has recently emerged as a mechanism underlying many nuclear compartments essential for cellular functions. Nuclear condensates enrich nucleic acids and proteins, localize to specific genomic regions, and often promote gene expression. How diverse properties of nuclear condensates are shaped by gene organization and activity is poorly understood. Here, we develop a physics-based model to interrogate how spatially-varying transcription activity impacts condensate properties and dynamics. Our model predicts that spatial clustering of active genes can enable precise localization and de novo nucleation of condensates. Strong clustering and high activity results in aspherical condensate morphologies. Condensates can flow towards distant gene clusters and competition between multiple clusters lead to stretched morphologies and activity-dependent repositioning. Overall, our model predicts and recapitulates morphological and dynamical features of diverse nuclear condensates and offers a unified mechanistic framework to study the interplay between non-equilibrium processes, spatially-varying transcription, and multicomponent condensates in cell biology.en_US
dc.language.isoen
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.isversionof10.1038/s41467-023-39878-4en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceSpringer Natureen_US
dc.titleA model for organization and regulation of nuclear condensates by gene activityen_US
dc.typeArticleen_US
dc.identifier.citationSchede, H.H., Natarajan, P., Chakraborty, A.K. et al. A model for organization and regulation of nuclear condensates by gene activity. Nat Commun 14, 4152 (2023).en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Institute for Medical Engineering & Scienceen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Physicsen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistryen_US
dc.relation.journalNature Communicationsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2024-12-09T20:56:45Z
dspace.orderedauthorsSchede, HH; Natarajan, P; Chakraborty, AK; Shrinivas, Ken_US
dspace.date.submission2024-12-09T20:56:46Z
mit.journal.volume14en_US
mit.journal.issue1en_US
mit.licensePUBLISHER_CC
mit.metadata.statusAuthority Work and Publication Information Neededen_US


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