Different gene regulation strategies revealed by analysis of binding motifs
Author(s)Mirny, Leonid A.; Wunderlich, Zeba
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Coordinated regulation of gene expression relies on transcription factors (TFs) binding to specific DNA sites. Our large-scale information-theoretic analysis of >950 TF-binding motifs demonstrates that prokaryotes and eukaryotes use strikingly different strategies to target TFs to specific genome locations. Although bacterial TFs can recognize a specific DNA site in the genomic background, eukaryotic TFs exhibit widespread, nonfunctional binding and require clustering of sites to achieve specificity. We find support for this mechanism in a range of experimental studies and in our evolutionary analysis of DNA-binding domains. Our systematic characterization of binding motifs provides a quantitative assessment of the differences in transcription regulation in prokaryotes and eukaryotes.
DepartmentHarvard University--MIT Division of Health Sciences and Technology
Trends in Genetics
Wunderlich, Zeba, and Leonid A. Mirny. “Different gene regulation strategies revealed by analysis of binding motifs.” Trends in Genetics 25.10 (2009): 434-440.
Author's final manuscript