Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells
Author(s)
Park, YongKeun; Yamauchi, Toyohiko; Choi, Wonshik; Dasari, Ramachandra Rao; Feld, Michael S.
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We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.
Date issued
2009-11Department
Harvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Physics; Massachusetts Institute of Technology. Spectroscopy LaboratoryJournal
Optics Letters
Publisher
Optical Society of America
Citation
YongKeun Park, Toyohiko Yamauchi, Wonshik Choi, Ramachandra Dasari, and Michael S. Feld, "Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells," Opt. Lett. 34, 3668-3670 (2009)
http://www.opticsinfobase.org/abstract.cfm?URI=ol-34-23-3668
Version: Author's final manuscript
ISSN
0146-9592