Production of Clostridium difficile toxin in a medium totally free of both animal and dairy proteins or digests
Author(s)
Demain, Arnold L.; Gerson, Donald F.; Fang, Aiqi
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In the hope of developing a vaccine against Clostridium difficile based on its toxin(s), we have developed a fermentation medium for the bacterium that results in the formation of Toxin A and contains no meat or dairy products, thus obviating the problem of possible prion diseases. Particular preparations of hydrolyzed soy proteins, especially Soy Peptone A3, have been found to replace both the meat/dairy product tryptone in the preparation of working cell banks and seed media, and NZ-Soy BL4 does the same in the fermentation medium. These replacements yield even higher toxin titers.
Date issued
2009-07Department
Massachusetts Institute of Technology. Department of BiologyJournal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
United States National Academy of Sciences
Citation
Fang, Aiqi, Donald F Gerson, and Arnold L Demain. “Production of Clostridium difficile toxin in a medium totally free of both animal and dairy proteins or digests.” Proceedings of the National Academy of Sciences 106.32 (2009): 13225-13229. © 2009 National Academy of Sciences
Version: Final published version
ISSN
0027-8424
1091-6490