dc.contributor.author | Brar, Gloria A. | |
dc.contributor.author | Ee, Ly-Sha | |
dc.contributor.author | Hochwagen, Andreas | |
dc.contributor.author | Amon, Angelika B | |
dc.date.accessioned | 2010-07-22T19:10:54Z | |
dc.date.available | 2010-07-22T19:10:54Z | |
dc.date.issued | 2009-02 | |
dc.date.submitted | 2008-11 | |
dc.identifier.issn | 1059-1524 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/57445 | |
dc.description.abstract | Sister chromatid cohesion, mediated by cohesin complexes, is laid down during DNA replication and is essential for the accurate segregation of chromosomes. Previous studies indicated that, in addition to their cohesion function, cohesins are essential for completion of recombination, pairing, meiotic chromosome axis formation, and assembly of the synaptonemal complex (SC). Using mutants in the cohesin subunit Rec8, in which phosphorylated residues were mutated to alanines, we show that cohesin phosphorylation is not only important for cohesin removal, but that cohesin's meiotic prophase functions are distinct from each other. We find pairing and SC formation to be dependent on Rec8, but independent of the presence of a sister chromatid and hence sister chromatid cohesion. We identified mutations in REC8 that differentially affect Rec8's cohesion, pairing, recombination, chromosome axis and SC assembly function. These findings define Rec8 as a key determinant of meiotic chromosome morphogenesis and a central player in multiple meiotic events. | en_US |
dc.description.sponsorship | United States National Institutes of Health (Grant GM62207) | en_US |
dc.description.sponsorship | National Science Foundation | en_US |
dc.language.iso | en_US | |
dc.publisher | American Society for Cell Biology | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1091/mbc.E08-06-0637 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | American Society of Cell Biology | en_US |
dc.title | The Multiple Roles of Cohesin in Meiotic Chromosome Morphogenesis and Pairing | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Brar, Gloria A. et al. “The Multiple Roles of Cohesin in Meiotic Chromosome Morphogenesis and Pairing.” Mol. Biol. Cell 20.3 (2009): 1030-1047. ©2009 by The American Society for Cell Biology. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
dc.contributor.approver | Amon, Angelika B. | |
dc.contributor.mitauthor | Amon, Angelika B. | |
dc.contributor.mitauthor | Brar, Gloria A. | |
dc.contributor.mitauthor | Ee, Ly-Sha | |
dc.contributor.mitauthor | Hochwagen, Andreas | |
dc.relation.journal | Molecular Biology of the Cell | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Brar, G. A.; Hochwagen, A.; Ee, L.-s. S.; Amon, A. | en |
dc.identifier.orcid | https://orcid.org/0000-0001-9837-0314 | |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |