Integrated Optical Coherence Tomography and Optical Coherence Microscopy Imaging of Human Pathology
Author(s)Lee, Hsiang-Chieh; Zhou, Chao; Wang, Yihong; Aguirre, Aaron Dominic; Tsai, Tsung-Han; Cohen, David W.; Connolly, James L.; Fujimoto, James G.; ... Show more Show less
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Excisional biopsy is the current gold standard for disease diagnosis; however, it requires a relatively long processing time and it may also suffer from unacceptable false negative rates due to sampling errors. Optical coherence tomography (OCT) is a promising imaging technique that provide real-time, high resolution and three-dimensional (3D) images of tissue morphology. Optical coherence microscopy (OCM) is an extension of OCT, combining both the coherence gating and the confocal gating techniques. OCM imaging achieves cellular resolution with deeper imaging depth compared to confocal microscopy. An integrated OCT/OCM imaging system can provide co-registered multiscale imaging of tissue morphology. 3D-OCT provides architectural information with a large field of view and can be used to find regions of interest; while OCM provides high magnification to enable cellular imaging. The integrated OCT/OCM system has an axial resolution of <4um and transverse resolutions of 14um and <2um for OCT and OCM, respectively. In this study, a wide range of human pathologic specimens, including colon (58), thyroid (43), breast (34), and kidney (19), were imaged with OCT and OCM within 2 to 6 hours after excision. The images were compared with H & E histology to identify characteristic features useful for disease diagnosis. The feasibility of visualizing human pathology using integrated OCT/OCM was demonstrated in the pathology laboratory settings.
DepartmentHarvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Research Laboratory of Electronics
Proceedings of SPIE--the International Society for Optical Engineering; v.7570
Lee, Hsiang-Chieh et al. “Integrated optical coherence tomography and optical coherence microscopy imaging of human pathology.” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII. Ed. Jose-Angel Conchello et al. San Francisco, California, USA: SPIE, 2010. 75700J-8. ©2010 COPYRIGHT SPIE--The International Society for Optical Engineering.
Final published version
Optical coherence tomography, optical coherence microscopy, human pathology