Comparison of cellular responses induced by low level light in different cell types
Author(s)Hamblin, Michael R.; Huang, Ying-Ying; Chen, Aaron Chih-Hao; Sharma, Sulbha K.; Wu, Qiuhe
MetadataShow full item record
Discoveries are rapidly being made in multiple laboratories that shed "light" on the fundamental molecular and cellular mechanisms underlying the use of low level light therapy (LLLT) in vitro, in animal models and in clinical practice. Increases in cellular levels of respiration, in cytochrome c oxidase activity, in ATP levels and in cyclic AMP have been found. Increased expression of reactive oxygen species and release of nitric oxide have also been shown. In order for these molecular changes to have a major effect on cell behavior, it is likely that various transcription factors will be activated, possibly via different signal transduction pathways. In this report we compare and contrast the effects of LLLT in vitro on murine embryonic fibroblasts, primary cortical neurons, cardiomyocytes and bone-marrow derived dendritic cells. We also examined two human cell lines, HeLa cancer cells and HaCaT keratinocytes. The effects of 810-nm near-infra-red light delivered at low and high fluences were addressed. Reactive oxygen species generation, transcription factor activation and ATP increases are reported. The data has led to the hypothesis that cells with a high level of mitochondrial activity (mitochondrial membrane potential) have a higher response to light than cells with low mitochondrial activity.
DepartmentHarvard University--MIT Division of Health Sciences and Technology
Proceedings of SPIE--the International Society for Optical Engineering
Society of Photo-optical Instrumentation Engineers
Huang, Ying-Ying et al. “Comparison of cellular responses induced by low level light in different cell types.” Mechanisms for Low-Light Therapy V. Ed. Michael R. Hamblin, Ronald W. Waynant, & Juanita Anders. San Francisco, California, USA: SPIE, 2010. 75520A-10. ©2010 SPIE--The International Society for Optical Engineering.
Final published version
Proc. of SPIE Vol. 7552, 75520A