dc.contributor.author | Rubins, Kathleen H. | |
dc.contributor.author | Hensley, Lisa E. | |
dc.contributor.author | Wahl-Jensen, Victoria | |
dc.contributor.author | Daddario DiCaprio, Kathleen M. | |
dc.contributor.author | Young, Howard | |
dc.contributor.author | Reed, Douglas S. | |
dc.contributor.author | Jahrling, Peter B. | |
dc.contributor.author | Brown, Patrick O. | |
dc.contributor.author | Relman, David A. | |
dc.contributor.author | Geisbert, Thomas W. | |
dc.date.accessioned | 2010-09-29T20:21:00Z | |
dc.date.available | 2010-09-29T20:21:00Z | |
dc.date.issued | 2007-08 | |
dc.date.submitted | 2007-02 | |
dc.identifier.issn | 1474-760X | |
dc.identifier.issn | 1465-6914 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/58769 | |
dc.description.abstract | Background: Infection with Ebola virus (EBOV) causes a fulminant and often fatal hemorrhagic fever. In order to improve our understanding of EBOV pathogenesis and EBOV-host interactions, we examined the molecular features of EBOV infection in vivo. Results: Using high-density cDNA microarrays, we analyzed genome-wide host expression patterns in sequential blood samples from nonhuman primates infected with EBOV. The temporal program of gene expression was strikingly similar between animals. Of particular interest were features of the data that reflect the interferon response, cytokine signaling, and apoptosis. Transcript levels for tumor necrosis factor-α converting enzyme (TACE)/α-disintegrin and metalloproteinase (ADAM)-17 increased during days 4 to 6 after infection. In addition, the serum concentration of cleaved Ebola glycoprotein (GP2 delta) was elevated in late-stage EBOV infected animals. Of note, we were able to detect changes in gene expression of more than 300 genes before symptoms appeared. Conclusion: These results provide the first genome-wide ex vivo analysis of the host response to systemic filovirus infection and disease. These data may elucidate mechanisms of viral pathogenesis and host defense, and may suggest targets for diagnostic and therapeutic development. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (NIH grant AI54922) | en_US |
dc.description.sponsorship | United States. Defense Advanced Research Projects Agency (DARPA grant N65236-99-1-5428) | en_US |
dc.description.sponsorship | Horn Foundation | en_US |
dc.description.sponsorship | Howard Hughes Medical Institute | en_US |
dc.description.sponsorship | United States. Defense Threat Reduction Agency | en_US |
dc.description.sponsorship | U.S. Army Medical Research Institute of Infectious Diseases | en_US |
dc.description.sponsorship | Chemical and Biological Defense Program (U.S.) | en_US |
dc.description.sponsorship | United States. Army Medical Research and Materiel Command (project number 02-4-4J-081) | en_US |
dc.publisher | BioMed Central Ltd | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1186/gb-2007-8-8-r174 | en_US |
dc.rights | Creative Commons Attribution | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0 | en_US |
dc.source | BioMed Central Ltd | en_US |
dc.title | The temporal program of peripheral blood gene expression in the response of non-human primates to Ebola hemorrhagic fever | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Genome Biology. 2007 Aug 28;8(8):R174 | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.mitauthor | Rubins, Kathleen H | |
dc.relation.journal | Genome Biology | en_US |
dc.eprint.version | Final published version | en_US |
dc.identifier.pmid | 17725815 | |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2010-09-03T16:19:26Z | |
dc.language.rfc3066 | en | |
dc.rights.holder | Rubins et al.; licensee BioMed Central Ltd. | |
dspace.orderedauthors | Rubins, Kathleen H; Hensley, Lisa E; Wahl-Jensen, Victoria; Daddario DiCaprio, Kathleen M; Young, Howard A; Reed, Douglas S; Jahrling, Peter B; Brown, Patrick O; Relman, David A; Geisbert, Thomas W | en |
dspace.mitauthor.error | true | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |