dc.contributor.author | Bortvin, Alex | |
dc.contributor.author | Goodheart, Mary L. | |
dc.contributor.author | Liao, Michelle | |
dc.contributor.author | Page, David C. | |
dc.date.accessioned | 2010-10-14T12:36:19Z | |
dc.date.available | 2010-10-14T12:36:19Z | |
dc.date.issued | 2004-02 | |
dc.date.submitted | 2003-12 | |
dc.identifier.issn | 1471-213X | |
dc.identifier.uri | http://hdl.handle.net/1721.1/59309 | |
dc.description.abstract | Background: In mice, germ cells are specified through signalling between layers of cells comprising the primitive embryo. The function of Dppa3 (also known as Pgc7 or stella), a gene expressed in primordial germ cells at the time of their emergence in gastrulating embryos, is unknown, but a recent study has claimed that it plays a central role in germ cell specification. Results: To test Dppa3's role in germ cell development, we disrupted the gene in mouse embryonic stem cells and generated mutant animals. We were able to obtain viable and fertile Dppa3-deficient animals of both sexes. Examination of embryonic and adult germ cells and gonads in Dppa3-deficient animals did not reveal any defects. However, most embryos derived from Dppa3-deficient oocytes failed to develop normally beyond the four-cell stage. Conclusion: We found that Dppa3 is an important maternal factor in the cleavage stages of mouse embryogenesis. However, it is not required for germ cell specification. | en_US |
dc.description.sponsorship | Howard Hughes Medical Institute | en_US |
dc.publisher | BioMed Central Ltd | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1186/1471-213X-4-2 | en_US |
dc.rights | Creative Commons Attribution | en_US |
dc.source | BioMed Central Ltd | en_US |
dc.subject | Animals | en_US |
dc.subject | Biological factors, physiology | en_US |
dc.subject | Cell differentiation, physiology | en_US |
dc.subject | Embryo, mammalian, cytology | en_US |
dc.subject | Embryo, mammalian, physiology | en_US |
dc.subject | Female | en_US |
dc.subject | Gene expression regulation, developmental, physiology | en_US |
dc.subject | Germ cells, physiology | en_US |
dc.subject | Gonads, cytology | en_US |
dc.subject | Gonads, physiology | en_US |
dc.subject | Male | en_US |
dc.subject | Mice | en_US |
dc.subject | Ovary, cytology | en_US |
dc.subject | Ovary, physiology | en_US |
dc.subject | Proteins, physiology | en_US |
dc.subject | Repressor proteins, physiology | en_US |
dc.subject | Biological factors | en_US |
dc.subject | Dppa3 protein, mouse | en_US |
dc.subject | Proteins | en_US |
dc.subject | Repressor proteins | en_US |
dc.title | Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Bortvin, Alex, Mary Goodheart, Michelle Liao, and David C. Page (2004). Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice. BMC developmental biology 4:2/1-15. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.mitauthor | Bortvin, Alex | |
dc.contributor.mitauthor | Goodheart, Mary L. | |
dc.contributor.mitauthor | Liao, Michelle | |
dc.contributor.mitauthor | Page, David C. | |
dc.relation.journal | BMC Developmental Biology | en_US |
dc.eprint.version | Final published version | en_US |
dc.identifier.pmid | 15018652 | |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2010-09-03T16:01:08Z | |
dc.language.rfc3066 | en | |
dc.rights.holder | Bortvin et al.; licensee BioMed Central Ltd. | |
dspace.orderedauthors | Bortvin, Alex; Goodheart, Mary; Liao, Michelle; Page, David C | en |
dc.identifier.orcid | https://orcid.org/0000-0001-9920-3411 | |
dspace.mitauthor.error | true | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |