Mutation K42R in Ribosomal Protein S12 Does Not Affect Susceptibility of Mycobacterium smegmatis 16S rRNA A-Site Mutants to 2-Deoxystreptamines

Author(s)
Hobbie, Sven N.; Kalapala, Sarath K.; Bottger, Erik C.; Shcherbakov, Dmitry
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Abstract
Recent studies have suggested that ribosomal protein S12 modulates 16S rRNA function and susceptibility to 2-deoxystreptamine aminoglycosides. To study whether the non-restrictive K42R mutation in RpsL affects 2-deoxystreptamine susceptibility in Mycobacterium smegmatis, we studied the drug susceptibility pattern of various mutants with genetic alterations in the 16S rRNA decoding A-site in the context of wild-type and mutant protein S12. RpsL K42R substitution was found not to affect the drug resistance pattern associated with mutational alterations in 16S rRNA H44.
Date issued
2010-04
URI
http://hdl.handle.net/1721.1/60365
Department
Singapore-MIT Alliance in Research and Technology (SMART)
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Kalapala, Sarath K. et al. “Mutation K42R in Ribosomal Protein S12 Does Not Affect Susceptibility of Mycobacterium smegmatis 16S rRNA A-Site Mutants to 2-Deoxystreptamines.” PLoS ONE 5.8 (2010): e11960.
Version: Final published version
ISSN
1932-6203
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