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Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus

Author(s)
Liu, Xiangan; Zhang, Qinfen; Murata, Kazuyoshi; Baker, Matthew L.; Sullivan, Matthew B.; Fu, Caroline; Dougherty, Matthew; Schmid, Michael F.; Osburne, Marcia; Chisholm, Sallie (Penny); Chiu, Wah; ... Show more Show less
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Abstract
Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single particle cryo-electron microscopy (cryo-EM) yields icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6 Å and 9 Å resolution, respectively. The asymmetric reconstruction reveals how symmetry mismatches are accommodated among 5 of the gene products at the portal vertex. Reconstructions of infectious and empty particles show a conformational change of the “valve” density in the nozzle, an orientation difference in the tail fibers, a disordering of the C-terminus of the portal protein, and disappearance of the core proteins. In addition, cryo-electron tomography (cryo-ET) of P-SSP7 infecting Prochlorococcus demonstrated the same tail fiber conformation as in empty particles. Our observations suggest a mechanism whereby, upon binding to the host cell, the tail fibers induce a cascade of structural alterations of the portal vertex complex that triggers DNA release.
Date issued
2010-06
URI
http://hdl.handle.net/1721.1/61294
Department
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
Journal
Nature Structural and Molecular Biology
Publisher
Nature Publishing Group
Citation
Liu, Xiangan et al. “Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus.” Nat Struct Mol Biol 17.7 (2010): 830-836.
Version: Author's final manuscript
ISSN
1545-9993
1545-9985

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