Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus
Author(s)Liu, Xiangan; Zhang, Qinfen; Murata, Kazuyoshi; Baker, Matthew L.; Sullivan, Matthew B.; Fu, Caroline; Dougherty, Matthew; Schmid, Michael F.; Osburne, Marcia; Chisholm, Sallie (Penny); Chiu, Wah; ... Show more Show less
MetadataShow full item record
Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single particle cryo-electron microscopy (cryo-EM) yields icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6 Å and 9 Å resolution, respectively. The asymmetric reconstruction reveals how symmetry mismatches are accommodated among 5 of the gene products at the portal vertex. Reconstructions of infectious and empty particles show a conformational change of the “valve” density in the nozzle, an orientation difference in the tail fibers, a disordering of the C-terminus of the portal protein, and disappearance of the core proteins. In addition, cryo-electron tomography (cryo-ET) of P-SSP7 infecting Prochlorococcus demonstrated the same tail fiber conformation as in empty particles. Our observations suggest a mechanism whereby, upon binding to the host cell, the tail fibers induce a cascade of structural alterations of the portal vertex complex that triggers DNA release.
DepartmentMassachusetts Institute of Technology. Department of Civil and Environmental Engineering
Nature Structural and Molecular Biology
Nature Publishing Group
Liu, Xiangan et al. “Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus.” Nat Struct Mol Biol 17.7 (2010): 830-836.
Author's final manuscript