Shack-Hartmann wavefront-sensor-based adaptive optics system for microscopy
Author(s)So, Peter T. C.; Cha, Jae Won; Ballesta, Jerome
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The imaging depth of two-photon excitation fluorescence microscopy is partly limited by the inhomogeneity of the refractive index in biological specimens. This inhomogeneity results in a distortion of the wavefront of the excitation light. This wavefront distortion results in image resolution degradation and lower signal level. Using an adaptive optics system consisting of a Shack-Hartmann wavefront sensor and a deformable mirror, wavefront distortion can be measured and corrected. With adaptive optics compensation, we demonstrate that the resolution and signal level can be better preserved at greater imaging depth in a variety of ex-vivo tissue specimens including mouse tongue muscle, heart muscle, and brain. However, for these highly scattering tissues, we find signal degradation due to scattering to be a more dominant factor than aberration.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering
Journal of Biomedical Optics
Cha, Jae Won, Jerome Ballesta, and Peter T. C. So. “Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy.” Journal of Biomedical Optics 15.4 (2010): 046022. © 2010 Society of Photo-Optical Instrumentation Engineers SPIE
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