Depth resolved wide field illumination for biomedical imaging and fabrication
Author(s)So, Peter T. C.; Kim, Daekeun
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Nonlinear microscopic imaging is relatively slow due to the sequential nature of raster scanning. Recently, this limitation was overcome by developing a 3D-resolved wide-field two-photon microscope based on the concept of temporal focusing. The existing temporal focusing systems have poor optical sectioning capability and, due to a shortage of illumination power, low actual frame rate. In this presentation, a comprehensive mathematical model for temporal focusing two-photon microscope will be presented. By optimizing instrument design and the use of high two-photon cross section quantum dots, we demonstrate single quantum dot imaging with submicron resolution at video rate and applied it to study transport processes in cells. Further, we realize that the depth resolved wide field illumination can be used for microfabrication. A prototype three-dimensional lithographic microfabrication system was developed and micropatterning capability based on photobleaching process is demonstrated.
DepartmentMassachusetts Institute of Technology. Department of Mechanical Engineering
31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2009. EMBC 2009
Institute of Electrical and Electronics Engineers
Siemens, M. et al. “EUV detection of high-frequency surface acoustic waves.” Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on. 2009. 1-2.© 2009 IEEE.
Author's final manuscript
INSPEC Accession Number: 10992455