Nanofabricated collagen-inspired synthetic elastomers for primary rat hepatocyte culture
Author(s)Bettinger, Christopher J.; Kulig, Katherine M.; Vacanti, Joseph P.; Langer, Robert; Borenstein, Jeffrey T.
MetadataShow full item record
Synthetic substrates that mimic the properties of extracellular matrix proteins hold significant promise for use in systems designed for tissue engineering applications. In this report, we designed a synthetic polymeric substrate that is intended to mimic chemical, mechanical, and topological characteristics of collagen. We found that elastomeric poly(ester amide) substrates modified with replica-molded nanotopographic features enhanced initial attachment, spreading, and adhesion of primary rat hepatocytes. Further, hepatocytes cultured on nanotopographic substrates also demonstrated reduced albumin secretion and urea synthesis, which is indicative of strongly adherent hepatocytes. These results suggest that these engineered substrates can function as synthetic collagen analogs for in vitro cell culture.
DepartmentCharles Stark Draper Laboratory; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Department of Materials Science and Engineering
Tissue Engineering. Part A
Mary Ann Liebert, Inc.
Bettinger, Christopher J. et al. “Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture.” Tissue Engineering Part A 15.6 (2009): 1321-1329. ©2009 Mary Ann Liebert, Inc.
Final published version