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dc.contributor.authorCho, Seung Woo
dc.contributor.authorKim, Il-Kwon
dc.contributor.authorKang, Jin Muk
dc.contributor.authorSong, Kang Won
dc.contributor.authorKim, Hong Sik
dc.contributor.authorPark, Chang Hwan
dc.contributor.authorYoo, Kyung Jong
dc.contributor.authorKim, Byung-Soo
dc.date.accessioned2011-03-11T21:05:48Z
dc.date.available2011-03-11T21:05:48Z
dc.date.issued2008-09
dc.identifier.issn1937-3341
dc.identifier.issn1937-335X
dc.identifier.urihttp://hdl.handle.net/1721.1/61684
dc.description.abstractNondegradable synthetic polymer vascular grafts currently used in cardiovascular surgery have no growth potential. Tissue-engineered vascular grafts (TEVGs) may solve this problem. In this study, we developed a TEVG using autologous bone marrow–derived cells (BMCs) and decellularized tissue matrices, and tested whether the TEVGs exhibit growth potential and vascular remodeling in vivo. Vascular smooth muscle–like cells and endothelial-like cells were differentiated from bone marrow mononuclear cells in vitro. TEVGs were fabricated by seeding these cells onto decellularized porcine abdominal aortas and implanted into the abdominal aortas of 4-month-old, bone marrow donor pigs (n¼4). Eighteen weeks after implantation, the dimensions of TEVGs were measured and compared with those of native abdominal aortas. Expression of molecules associated with vascular remodeling was examined with reverse transcription–polymerase chain reaction assay and immunohistochemistry. Eighteen weeks after implantation, all TEVGs were patent with no sign of thrombus formation, dilatation, or stenosis. Histological and immunohistochemical analyses of the retrieved TEVGs revealed regeneration of endothelium and smooth muscle and the presence of collagen and elastin. The outer diameter of three of the four TEVGs increased in proportion to increases in body weight and outer native aorta diameter. Considerable extents of expression of molecules associated with extracellular matrix (ECM) degradation (i.e., matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase) and ECM precursors (i.e., procollagen I, procollagen III, and tropoelastin) occurred in the TEVGs, indicating vascular remodeling associated with degradation of exogenous ECMs (implanted decellularized matrices) and synthesis of autologous ECMs. This study demonstrates that the TEVGs with autologous BMCs and decellularized tissue matrices exhibit growth potential and vascular remodeling in vivo of tissue-engineered artery.en_US
dc.description.sponsorshipKorea. Ministry of Health and Welfare (Grant A050082) (Grant 02-PJ10- PG8-EC01-0016)en_US
dc.language.isoen_US
dc.publisherMary Ann Lieberten_US
dc.relation.isversionofhttp://dx.doi.org/10.1089/ten.tea.2008.0172en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceMary Ann Lieberten_US
dc.titleEvidence for In Vivo Growth Potential and Vascular Remodeling of Tissue-Engineered Arteryen_US
dc.typeArticleen_US
dc.identifier.citationCho, Seung-Woo et al. "Evidence for <i>In Vivo</i> Growth Potential and Vascular Remodeling of Tissue-Engineered Artery." Tissue Engineering Part A 15.4 (2009): 901-912. © 2009, Mary Ann Liebert, Inc.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.approverCho, Seung Woo
dc.contributor.mitauthorCho, Seung Woo
dc.relation.journalTissue Engineering. Part Aen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsCho, Seung-Woo; Kim, Il-Kwon; Kang, Jin Muk; Song, Kang Won; Kim, Hong Sik; Park, Chang Hwan; Yoo, Kyung Jong; Kim, Byung-Sooen
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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