dc.contributor.author | Thomson, John M. | |
dc.contributor.author | Hansen, Richard | |
dc.contributor.author | Berry, Susan H. | |
dc.contributor.author | Hope, Mairi E. | |
dc.contributor.author | Murray, Graeme I. | |
dc.contributor.author | Mukhopadhya, Indrani | |
dc.contributor.author | McLean, Mairi H. | |
dc.contributor.author | Shen, Zeli | |
dc.contributor.author | Fox, James G. | |
dc.contributor.author | El-Omar, Emad | |
dc.contributor.author | Hold, Georgina L. | |
dc.date.accessioned | 2011-06-22T21:05:09Z | |
dc.date.available | 2011-06-22T21:05:09Z | |
dc.date.issued | 2011-02 | |
dc.date.submitted | 2010-09 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/64658 | |
dc.description.abstract | Background
Changes in bacterial populations termed “dysbiosis” are thought central to ulcerative colitis (UC) pathogenesis. In particular, the possibility that novel Helicobacter organisms play a role in human UC has been debated but not comprehensively investigated. The aim of this study was to develop a molecular approach to investigate the presence of Helicobacter organisms in adults with and without UC.
Methodology/Principal Findings
A dual molecular approach to detect Helicobacter was developed. Oligonucleotide probes against the genus Helicobacter were designed and optimised alongside a validation of published H. pylori probes. A comprehensive evaluation of Helicobacter genus and H. pylori PCR primers was also undertaken. The combined approach was then assessed in a range of gastrointestinal samples prior to assessment of a UC cohort. Archival colonic samples were available from 106 individuals for FISH analysis (57 with UC and 49 non-IBD controls). A further 118 individuals were collected prospectively for dual FISH and PCR analysis (86 UC and 32 non-IBD controls). An additional 27 non-IBD controls were available for PCR analysis. All Helicobacter PCR-positive samples were sequenced. The association between Helicobacter and each study group was statistically analysed using the Pearson Chi Squared 2 tailed test. Helicobacter genus PCR positivity was significantly higher in UC than controls (32 of 77 versus 11 of 59, p = 0.004). Sequence analysis indicated enterohepatic Helicobacter species prevalence was significantly higher in the UC group compared to the control group (30 of 77 versus 2 of 59, p<0.0001). PCR and FISH results were concordant in 74 (67.9%) of subjects. The majority of discordant results were attributable to a higher positivity rate with FISH than PCR.
Conclusions/Significance
Helicobacter organisms warrant consideration as potential pathogenic entities in UC. Isolation of these organisms from colonic tissue is needed to enable interrogation of pathogenicity against established criteria. | en_US |
dc.description.sponsorship | Broad Medical Research Program (Grant number IBD-0178) | en_US |
dc.description.sponsorship | Aberdeen Royal Infirmary. GI Research Unit | en_US |
dc.description.sponsorship | NHS Scotland (Clinical Academic Training Fellowship, from the Chief Scientist Office (CAF/08/01)) | en_US |
dc.language.iso | en_US | |
dc.publisher | Public Library of Science | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1371/journal.pone.0017184 | en_US |
dc.rights | Creative Commons Attribution | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/2.5/ | en_US |
dc.source | PLoS | en_US |
dc.title | Enterohepatic Helicobacter in Ulcerative Colitis: Potential Pathogenic Entities? | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Thomson, John M. et al "Enterohepatic Helicobacter in Ulcerative Colitis: Potential Pathogenic Entities?" PLoS ONE 6(2): e17184. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Division of Comparative Medicine | en_US |
dc.contributor.approver | Fox, James G. | |
dc.contributor.mitauthor | Fox, James G. | |
dc.relation.journal | PLoS ONE | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Thomson, John M.; Hansen, Richard; Berry, Susan H.; Hope, Mairi E.; Murray, Graeme I.; Mukhopadhya, Indrani; McLean, Mairi H.; Shen, Zeli; Fox, James G.; El-Omar, Emad; Hold, Georgina L. | en |
dc.identifier.orcid | https://orcid.org/0000-0001-9307-6116 | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |