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dc.contributor.authorTodd, Ryan C.
dc.contributor.authorLippard, Stephen J.
dc.date.accessioned2011-07-07T18:40:21Z
dc.date.available2011-07-07T18:40:21Z
dc.date.issued2010-04
dc.date.submitted2010-04
dc.identifier.issn0162-0134
dc.identifier.urihttp://hdl.handle.net/1721.1/64764
dc.description.abstractWe report the 1.77-Å resolution X-ray crystal structure of a dodecamer DNA duplex with the sequence 5′-CCTCTGGTCTCC-3′ that has been modified to contain a single engineered 1,2-cis-{Pt(NH3)2}2+-d(GpG) cross-link, the major DNA adduct of cisplatin. These data represent a significant improvement in resolution over the previously published 2.6-Å structure. The ammine ligands in this structure are clearly resolved, leading to improved visualization of the cross-link geometry with respect to both the platinum center and to the nucleobases, which adopt a higher energy conformation. Also better resolved are the deoxyribose sugar puckers, which allow us to re-examine the global structure of platinum-modified DNA. Another new feature of this model is the location of four octahedral [Mg(H2O)6]2+ ions associated with bases in the DNA major groove and the identification of 124 ordered water molecules that participate in hydrogen-bonding interactions with either the nucleic acid or the diammineplatinum(II) moiety.en_US
dc.description.sponsorshipNational Cancer Institute (U.S.) (grant CA034992)en_US
dc.description.sponsorshipDavid H. Koch Institute for Integrative Cancer Research at MIT (Koch Fund Fellowship)en_US
dc.language.isoen_US
dc.publisherElsevier B.V.en_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.jinorgbio.2010.04.005en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourceProf. Lippard via Erja Kajosaloen_US
dc.titleStructure of duplex DNA containing the cisplatin 1,2-{Pt(NH3)2}[superscript 2]+-d(GpG) crosslink at 1.77 [Angstrom] resolutionen_US
dc.title.alternativeStructure of duplex DNA containing the cisplatin 1,2-{Pt(NH3)2}2+-d(GpG) crosslink at 1.77 Å resolutionen_US
dc.typeArticleen_US
dc.identifier.citationTodd, Ryan C., and Stephen J. Lippard. “Structure of Duplex DNA Containing the Cisplatin 1,2-{Pt(NH3)2}[superscript 2+]-d(GpG) Cross-link at 1.77[Ångstrom] Resolution.” Journal of Inorganic Biochemistry 104.9 (2010) : 902-908.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistryen_US
dc.contributor.approverLippard, Stephen J.
dc.contributor.mitauthorLippard, Stephen J.
dc.contributor.mitauthorTodd, Ryan C.
dc.relation.journalJournal of Inorganic Biochemistryen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsTodd, Ryan C.; Lippard, Stephen J.en
dc.identifier.orcidhttps://orcid.org/0000-0002-2693-4982
mit.licenseOPEN_ACCESS_POLICYen_US


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