Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human
Author(s)Venkatachalam, Gireedhar; Kumar, Alan Prem; Yue, Loo Ser; Pervaiz, Shazib; Clement, Marie Veronique; Sakharkar, Meena Kishore; ... Show more Show less
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Background: Activation of PPARs has been reported to inhibit the proliferation of malignant cells from different lineages. They are involved in transcription regulation of genes upon activation by a ligand. The binding of PPARs to the promoter sequence either represses or activates the gene. Hence, PPARs represent promising targets for cancer treatment because of their anti-proliferative and pro-apoptotic activities. Here we computationally identified PPAR binding regions in NHE1 and MnSOD. We further validated the predictions in vitro. Results: Our results computationally predicted the presence of 2 PPRE motifs in NHE1 and 3 PPRE motifs in MnSOD. We experimentally confirmed the true motifs and their regulation by PPAR. Conclusion: Our results suggest that both NHE1 and MnSOD have PPRE binding motif in their upstream/promoter region and hence are regulated by PPAR upon ligand binding.
DepartmentSingapore-MIT Alliance in Research and Technology (SMART)
Venkatachalam, Gireedhar et al. “Computational Identification and Experimental Validation of PPRE Motifs in NHE1 and MnSOD Genes of Human.” BMC Genomics 10.Suppl 3 (2009) : S5. © 2009 Venkatachalam et al; licensee BioMed Central Ltd.
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