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dc.contributor.authorKlaas Smits, Wiep
dc.contributor.authorGrossman, Alan Davis
dc.date.accessioned2011-07-13T15:52:21Z
dc.date.available2011-07-13T15:52:21Z
dc.date.issued2010-11
dc.date.submitted2010-08
dc.identifier.issn1553-7404
dc.identifier.issn1553-7390
dc.identifier.urihttp://hdl.handle.net/1721.1/64787
dc.description.abstractThe rok gene of Bacillus subtilis was identified as a negative regulator of competence development. It also controls expression of several genes not related to competence. We found that Rok binds to extended regions of the B. subtilis genome. These regions are characterized by a high A+T content and are known or believed to have been acquired by horizontal gene transfer. Some of the Rok binding regions are in known mobile genetic elements. A deletion of rok resulted in higher excision of one such element, ICEBs1, a conjugative transposon found integrated in the B. subtilis genome. When expressed in the Gram negative E. coli, Rok also associated with A+T-rich DNA and a conserved C-terminal region of Rok contributed to this association. Together with previous work, our findings indicate that Rok is a nucleoid associated protein that serves to help repress expression of A+T-rich genes, many of which appear to have been acquired by horizontal gene transfer. In these ways, Rok appears to be functionally analogous to H-NS, a nucleoid associated protein found in Gram negative bacteria and Lsr2 of high G+C Mycobacteria.en_US
dc.description.sponsorshipNetherlands Organization for Scientific Research (Rubicon fellowship (file number 825.07.033)en_US
dc.description.sponsorshipUnited States. Public Health Service (grant GM41934)en_US
dc.description.sponsorshipUnited States. Public Health Service (grant GM50895)en_US
dc.language.isoen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pgen.1001207en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/en_US
dc.sourcePLoSen_US
dc.titleThe Transcriptional Regulator Rok Binds A plus T-Rich DNA and Is Involved in Repression of a Mobile Genetic Element in Bacillus subtilisen_US
dc.typeArticleen_US
dc.identifier.citationSmits, Wiep Klaas, Alan D. Grossman. "The Transcriptional Regulator Rok Binds A+T-Rich DNA and Is Involved in Repression of a Mobile Genetic Element in Bacillus subtilis." PLoS Genetics (2010) 6(11): e1001207.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.approverGrossman, Alan D.
dc.contributor.mitauthorGrossman, Alan D.
dc.relation.journalPLoS Geneticsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsSmits, Wiep Klaas; Grossman, Alan D.en
dc.identifier.orcidhttps://orcid.org/0000-0002-8235-7227
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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