dc.contributor.author | Fortune, Jennifer A. | |
dc.contributor.author | Novobrantseva, Tatiana I. | |
dc.contributor.author | Klibanov, Alexander M. | |
dc.date.accessioned | 2011-07-13T19:01:44Z | |
dc.date.available | 2011-07-13T19:01:44Z | |
dc.date.issued | 2011-01 | |
dc.date.submitted | 2011-01 | |
dc.identifier.issn | 2090-3022 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/64795 | |
dc.description.abstract | We mechanistically explored the effect of increased hydrophobicity of the polycation on the efficacy and specificity of gene delivery in mice. N-Alkylated linear PEIs with varying alkyl chain lengths and extent of substitution were synthesized and characterized by biophysical methods. Their in vivo transfection efficiency, specificity, and biodistribution were investigated. N-Ethylation improves the in vivo efficacy of gene expression in the mouse lung 26-fold relative to the parent polycation and more than quadruples the ratio of expression in the lung to that in all other organs. N-Propyl-PEI was the best performer in the liver and heart (581- and 3.5-fold enhancements, resp.) while N-octyl-PEI improved expression in the kidneys over the parent polymer 221-fold. As these enhancements in gene expression occur without changing the plasmid biodistribution, alkylation does not alter the cellular uptake but rather enhances transfection subsequent to cellular uptake. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (NIH Grant 2-R37-EB000244-29) | en_US |
dc.description.sponsorship | National Science Foundation (U.S.) (NSF-0070319 ) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (NIH GM68762) | en_US |
dc.language.iso | en_US | |
dc.publisher | Hindawi Pub. Corp. | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1155/2011/204058 | en_US |
dc.rights | Creative Commons Attribution | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0/ | en_US |
dc.source | Hindawi | en_US |
dc.title | Highly Effective Gene Transfection In Vivo by Alkylated Polyethylenimine | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Jennifer A. Fortune, Tatiana I. Novobrantseva, and Alexander M. Klibanov, “Highly Effective Gene Transfection In Vivo by Alkylated Polyethylenimine,” Journal of Drug Delivery, vol. 2011, Article ID 204058, 6 pages, 2011. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
dc.contributor.approver | Klibanov, Alexander M | |
dc.contributor.mitauthor | Fortune, Jennifer A. | |
dc.contributor.mitauthor | Klibanov, Alexander M. | |
dc.relation.journal | Journal of Drug Delivery | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Fortune, Jennifer A.; Novobrantseva, Tatiana I.; Klibanov, Alexander M. | en |
dc.identifier.orcid | https://orcid.org/0000-0003-3830-714X | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |