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dc.contributor.authorFortune, Jennifer A.
dc.contributor.authorNovobrantseva, Tatiana I.
dc.contributor.authorKlibanov, Alexander M.
dc.date.accessioned2011-07-13T19:01:44Z
dc.date.available2011-07-13T19:01:44Z
dc.date.issued2011-01
dc.date.submitted2011-01
dc.identifier.issn2090-3022
dc.identifier.urihttp://hdl.handle.net/1721.1/64795
dc.description.abstractWe mechanistically explored the effect of increased hydrophobicity of the polycation on the efficacy and specificity of gene delivery in mice. N-Alkylated linear PEIs with varying alkyl chain lengths and extent of substitution were synthesized and characterized by biophysical methods. Their in vivo transfection efficiency, specificity, and biodistribution were investigated. N-Ethylation improves the in vivo efficacy of gene expression in the mouse lung 26-fold relative to the parent polycation and more than quadruples the ratio of expression in the lung to that in all other organs. N-Propyl-PEI was the best performer in the liver and heart (581- and 3.5-fold enhancements, resp.) while N-octyl-PEI improved expression in the kidneys over the parent polymer 221-fold. As these enhancements in gene expression occur without changing the plasmid biodistribution, alkylation does not alter the cellular uptake but rather enhances transfection subsequent to cellular uptake.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH Grant 2-R37-EB000244-29)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (NSF-0070319 )en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH GM68762)en_US
dc.language.isoen_US
dc.publisherHindawi Pub. Corp.en_US
dc.relation.isversionofhttp://dx.doi.org/10.1155/2011/204058en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/en_US
dc.sourceHindawien_US
dc.titleHighly Effective Gene Transfection In Vivo by Alkylated Polyethylenimineen_US
dc.typeArticleen_US
dc.identifier.citationJennifer A. Fortune, Tatiana I. Novobrantseva, and Alexander M. Klibanov, “Highly Effective Gene Transfection In Vivo by Alkylated Polyethylenimine,” Journal of Drug Delivery, vol. 2011, Article ID 204058, 6 pages, 2011.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistryen_US
dc.contributor.approverKlibanov, Alexander M
dc.contributor.mitauthorFortune, Jennifer A.
dc.contributor.mitauthorKlibanov, Alexander M.
dc.relation.journalJournal of Drug Deliveryen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsFortune, Jennifer A.; Novobrantseva, Tatiana I.; Klibanov, Alexander M.en
dc.identifier.orcidhttps://orcid.org/0000-0003-3830-714X
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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