Synthesis and Characterization of Tunable PEG - Gelatin Methacrylate Hydrogels
Name
Hutson-2011-Synthesis and Characterization of Tunable PEG - Gelatin Methacrylate Hydrogels.pdf
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969.59 KB
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Author(s)
Hutson, Che B.
Nichol, Jason W.
Aubin, Hug
Bae, Hojae
Yamanlar, Seda
Al-Haque, Mohd. Shahed
Koshy, Sandeep Tharian
Khademhosseini, Ali
Date Issued
April 2011
Journal
Tissue Engineering, Part A.
Publisher
Mary Ann Liebert
Citation
Hutson, Che B. et al. “Synthesis and Characterization of Tunable Poly(Ethylene Glycol): Gelatin Methacrylate Composite Hydrogels.” Tissue Engineering Part A 17.13-14 (2011) : 1713-1723.
Copyright © 2011, Mary Ann Liebert, Inc. publishers
Version
Final published version
Abstract
Poly(ethylene glycol) (PEG) hydrogels are popular for cell culture and tissue-engineering applications because they are nontoxic and exhibit favorable hydration and nutrient transport properties. However, cells cannot adhere to, remodel, proliferate within, or degrade PEG hydrogels. Methacrylated gelatin (GelMA), derived from denatured collagen, yields an enzymatically degradable, photocrosslinkable hydrogel that cells can degrade, adhere to and spread within. To combine the desirable features of each of these materials we synthesized PEG-GelMA composite hydrogels, hypothesizing that copolymerization would enable adjustable cell binding, mechanical, and degradation properties. The addition of GelMA to PEG resulted in a composite hydrogel that exhibited tunable mechanical and biological profiles. Adding GelMA (5%–15% w/v) to PEG (5% and 10% w/v) proportionally increased fibroblast surface binding and spreading as compared to PEG hydrogels (p<0.05). Encapsulated fibroblasts were also able to form 3D cellular networks 7 days after photoencapsulation only within composite hydrogels as compared to PEG alone. Additionally, PEG-GelMA hydrogels displayed tunable enzymatic degradation and stiffness profiles. PEG-GelMA composite hydrogels show great promise as tunable, cell-responsive hydrogels for 3D cell culture and regenerative medicine applications.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1089/ten.TEA.2010.0666
