MIT Libraries logoDSpace@MIT

MIT
View Item 
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Integrin alpha 5 beta 1 is necessary for regulation of radial migration of cortical neurons during mouse brain development

Author(s)
Marchetti, Giovanni; Escuin, Sarah; van der Flier, Arjan; De Arcangelis, Adele; Georges-Labouesse, Elisabeth; Hynes, Richard O; ... Show more Show less
Thumbnail
DownloadHynes_Integrin alpha.pdf (1.456Mb)
PUBLISHER_POLICY

Publisher Policy

Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.

Alternative title
Integrin α5β1 is necessary for regulation of radial migration of cortical neurons during mouse brain development
Terms of use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Metadata
Show full item record
Abstract
During cerebral cortex development, post-mitotic neurons interact with radial glial fibers and the extracellular environment to migrate away from the ventricular region and form a correct laminar structure. Integrin receptors are major mediators of cell–cell and cell–extracellular matrix interactions. Several integrin heterodimers are present during formation of the cortical layers. The α5β1 receptor is expressed in the neural progenitors of the ventricular zone during cerebral cortex formation. Using in utero electroporation to introduce short hairpin RNAs in the brain at embryonic day 15.5, we were able to inhibit acutely the expression of α5 integrin in the developing cortex. The knockdown of α5 integrin expression level in neural precursors resulted in an inhibition of radial migration, without perturbing the glial scaffold. Moreover, the same inhibitory effect on neuronal migration was observed after electroporation of a Cre recombinase expression plasmid into the neural progenitors of conditional knockout mice for α5 integrin. In both types of experiments, the electroporated cells expressing reduced levels of α5 integrin accumulated in the premigratory region with an abnormal morphology. At postnatal day 2, ectopic neurons were observed in cortical layer V, while a deficit of neurons was observed in cortical layer II–IV. We show that these neurons do not express a layer V-specific marker, suggesting that they have not undergone premature differentiation. Overall, these results indicate that α5β1 integrin functions in the regulation of neural morphology and migration during cortical development, playing a role in cortical lamination.
Date issued
2010-02
URI
http://hdl.handle.net/1721.1/65110
Department
Massachusetts Institute of Technology. Department of Biology; Koch Institute for Integrative Cancer Research at MIT
Journal
European Journal of Neuroscience
Publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
Citation
Marchetti, Giovanni et al. “Integrin Α5β1 Is Necessary for Regulation of Radial Migration of Cortical Neurons During Mouse Brain Development.” European Journal of Neuroscience 31.3 (2010) : 399-409.
Version: Final published version
ISSN
0953-816X
1460-9584

Collections
  • MIT Open Access Articles

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

Login

Statistics

OA StatisticsStatistics by CountryStatistics by Department
MIT Libraries
PrivacyPermissionsAccessibilityContact us
MIT
Content created by the MIT Libraries, CC BY-NC unless otherwise noted. Notify us about copyright concerns.