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dc.contributor.authorde Puig Guixe, Helena
dc.contributor.authorFederici, Stefania
dc.contributor.authorBaxamusa, Salmaan H.
dc.contributor.authorBergese, Paolo
dc.contributor.authorHamad-Schifferli, Kimberly
dc.date.accessioned2011-09-12T21:28:09Z
dc.date.available2011-09-12T21:28:09Z
dc.date.issued2011-06
dc.identifier.issn1613-6810
dc.identifier.urihttp://hdl.handle.net/1721.1/65656
dc.description.abstractA study is presented of the nanomechanical phenomena experienced by nanoparticle-conjugated biomolecules. A thermodynamic framework is developed to describe the binding of thrombin-binding aptamer (TBA) to thrombin when the TBA is conjugated to nanorods. Binding results in nanorod aggregation (viz. directed self-assembly), which is detectable by absorption spectroscopy. The analysis introduces the energy of aggregation, separating it into TBA–thrombin recognition and surface-work contributions. Consequently, it is demonstrated that self-assembly is driven by the interplay of surface work and thrombin-TBA recognition. It is shown that the work at the surface is about −10 kJ mol−1 [mol superscript -1] and results from the accumulation of in-plane molecular forces of pN magnitude and with a lifetime of <1 s, which arises from TBA nanoscale rearrangements fuelled by thrombin-directed nanorod aggregation. The obtained surface work can map aggregation regimes as a function of different nanoparticle surface conditions. Also, the thermodynamic treatment can be used to obtain quantitative information on surface effects impacting biomolecules on nanoparticle surfaces.en_US
dc.description.sponsorshipMIT-IQS Exchange Programen_US
dc.language.isoen_US
dc.publisherWiley-VCHen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/smll.201100530en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourceProf. Hamad-Schifferlien_US
dc.titleQuantifying the nanomachinery of the nanoparticle-biomolecule interfaceen_US
dc.typeArticleen_US
dc.identifier.citationde Puig, Helena et al. “Quantifying the Nanomachinery of the Nanoparticle-Biomolecule Interface.” Small (2011) : v. 7, issue 17, p. 2477-2484, Sept. 2011. Copyright © 2011 WILEY-VCH Verlag GmbH & Co.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.approverHamad-Schifferli, Kimberly
dc.contributor.mitauthorde Puig Guixe, Helena
dc.contributor.mitauthorFederici, Stefania
dc.contributor.mitauthorBaxamusa, Salmaan H.
dc.contributor.mitauthorBergese, Paolo
dc.contributor.mitauthorHamad-Schifferli, Kimberly
dc.relation.journalSmallen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsde Puig, Helena; Federici, Stefania; Baxamusa, Salmaan H.; Bergese, Paolo; Hamad-Schifferli, Kimberlyen
dc.identifier.orcidhttps://orcid.org/0000-0002-5368-6996
dspace.mitauthor.errortrue
mit.licenseOPEN_ACCESS_POLICYen_US


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