MIT Libraries logoDSpace@MIT

MIT
View Item 
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo

Author(s)
Waite, Janelle C.; Leiner, Ingrid; Lauer, Peter; Rae, Chris S.; Barbet, Gaetan; Zheng, Huan; Portnoy, Daniel A.; Pamer, Eric G.; Dustin, Michael L.; ... Show more Show less
Thumbnail
DownloadWaite-2011-Dynamic Imaging of t.pdf (7.546Mb)
PUBLISHER_CC

Publisher with Creative Commons License

Creative Commons Attribution

Terms of use
Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/
Metadata
Show full item record
Abstract
Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated with scDC. Myelomonocytic cells (MMC) swarmed around non-motile scDC forming foci from which blood flow was excluded. The depletion of scDC after foci were established resulted in a 10-fold reduction in viable Lm, while graded depletion of MMC resulted in 30–1000 fold increase in viable Lm in foci with enhanced blood flow. Effector CD8+ [CD8 superscript +] T cells at sites of infection displayed a two-tiered reduction in motility with antigen independent and antigen dependent components, including stable interactions with infected and non-infected scDC. Thus, swarming MMC contribute to control of Lm prior to development of T cell immunity by direct killing and sequestration from blood flow, while scDC appear to promote Lm survival while preferentially interacting with CD8+ [CD8 superscript +] T cells in effector sites.
Date issued
2011-03
URI
http://hdl.handle.net/1721.1/66113
Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Journal
PLoS Pathogens
Publisher
Public Library of Science
Citation
Waite, Janelle C. et al. “Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo.” Ed. David S. Schneider. PLoS Pathogens 7 (3) (2011): e1001326. © 2011 Waite et al.
Version: Final published version
ISSN
1553-7366
1553-7374

Collections
  • MIT Open Access Articles

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

Login

Statistics

OA StatisticsStatistics by CountryStatistics by Department
MIT Libraries
PrivacyPermissionsAccessibilityContact us
MIT
Content created by the MIT Libraries, CC BY-NC unless otherwise noted. Notify us about copyright concerns.