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dc.date.accessioned2011-10-26T18:15:40Z
dc.date.available2011-10-26T18:15:40Z
dc.date.issued2010-02
dc.identifier.issn0021-9290
dc.identifier.urihttp://hdl.handle.net/1721.1/66592
dc.description.abstractThe success of cell-based tissue engineering approaches in restoring biological function will be facilitated by a comprehensive fundamental knowledge of the temporal evolution of the structure and properties of the newly synthesized matrix. Here, we quantify the dynamic oscillatory mechanical behavior of the engineered matrix associated with individual chondrocytes cultured in vitro for up to 28 days in alginate scaffolds. The magnitude of the complex modulus (|E*|) and phase shift (δ) were measured in culture medium using Atomic Force Microscopy (AFM)-based nanoindentation in response to an imposed oscillatory deformation (amplitude ∼5 nm) as a function of frequency (f=1–316 Hz), probe tip geometry (2.5 μm radius sphere and 50 nm radius square pyramid), and in the absence and presence of growth factors (GF, insulin growth factor-1, IGF-1, and osteogenic protein-1, OP-1). |E*| for all conditions increased nonlinearly with frequency dependence approximately f[superscript 1/2] and ranged between ∼1 and 25 kPa. This result, along with theoretical calculations of the characteristic poroelastic relaxation frequency, f[subscript p], (∼50–90 Hz) suggested that this time-dependent behavior was governed primarily by fluid flow-dependent poroelasticity, rather than flow-independent viscoelastic processes associated with the solid matrix. |E*(f)| increased, (f) decreased, and the hydraulic permeability, k, decreased with time in culture and with growth factor treatment. This trend of a more elastic-like response was thought to be associated with increased macromolecular biosynthesis, density, and a more mature matrix structure/organization.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.). Nanoscale Interdisciplinary Research Teams (0403903)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.). Civil, Mechanical and Manufacturing Innovation (0758651)en_US
dc.description.sponsorshipNational Institute of Mental Health (U.S.) (grant AR33236)en_US
dc.description.sponsorshipMassachusetts Institute of Technology. Institute for Soldier Nanotechnologies (contract number DAAD-19-02-D0002)en_US
dc.language.isoen_US
dc.publisherElsevier Ltd.en_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.jbiomech.2009.09.053en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourcePubMed Centralen_US
dc.titleDynamic mechanical properties of the tissue-engineered matrix associated with individual chondrocytesen_US
dc.typeArticleen_US
dc.identifier.citationLee, BoBae et al. “Dynamic mechanical properties of the tissue-engineered matrix associated with individual chondrocytes.” Journal of Biomechanics 43 (2010): 469-476. Web. 26 Oct. 2011. © 2010 Elsevier Ltd.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Materials Science and Engineering
dc.contributor.departmentMassachusetts Institute of Technology. Center for Biomedical Engineering
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineering
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineering
dc.contributor.approverGrodzinsky, Alan J.
dc.contributor.mitauthorLee, BoBae
dc.contributor.mitauthorHan, Lin
dc.contributor.mitauthorFrank, Eliot H.
dc.contributor.mitauthorOrtiz, Christine
dc.contributor.mitauthorGrodzinsky, Alan J.
dc.relation.journalJournal of Biomechanicsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsLee, BoBae; Han, Lin; Frank, Eliot H.; Chubinskaya, Susan; Ortiz, Christine; Grodzinsky, Alan J.en
dc.identifier.orcidhttps://orcid.org/0000-0003-3511-5679
dc.identifier.orcidhttps://orcid.org/0000-0002-4942-3456
dspace.mitauthor.errortrue
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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