dc.contributor.author | Chen, Xiaobing | |
dc.contributor.author | Nelson, Christopher D. | |
dc.contributor.author | Li, Xiang | |
dc.contributor.author | Winters, Christine A. | |
dc.contributor.author | Azzam, Rita | |
dc.contributor.author | Sousa, Alioscka A. | |
dc.contributor.author | Leapman, Richard D. | |
dc.contributor.author | Gainer, Harold | |
dc.contributor.author | Sheng, Morgan Hwa-Tze | |
dc.contributor.author | Reese, Thomas S. | |
dc.date.accessioned | 2011-11-09T16:01:08Z | |
dc.date.available | 2011-11-09T16:01:08Z | |
dc.date.issued | 2011-03 | |
dc.date.submitted | 2011-03 | |
dc.identifier.issn | 0270-6474 | |
dc.identifier.issn | 1529-2401 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/66976 | |
dc.description.abstract | PSD-95, a membrane-associated guanylate kinase, is the major scaffolding protein in the excitatory postsynaptic density (PSD) and a potent regulator of synaptic strength. Here we show that PSD-95 is in an extended configuration and positioned into regular arrays of vertical filaments that contact both glutamate receptors and orthogonal horizontal elements layered deep inside the PSD in rat hippocampal spine synapses. RNA interference knockdown of PSD-95 leads to loss of entire patches of PSD material, and electron microscopy tomography shows that the patchy loss correlates with loss of PSD-95-containing vertical filaments, horizontal elements associated with the vertical filaments, and putative AMPA receptor-type, but not NMDA receptor-type, structures. These observations show that the orthogonal molecular scaffold constructed from PSD-95-containing vertical filaments and their associated horizontal elements is essential for sustaining the three-dimensional molecular organization of the PSD. Our findings provide a structural basis for understanding the functional role of PSD-95 at the PSD. | en_US |
dc.description.sponsorship | National Institute of Neurological Disorders and Stroke (U.S.) | en_US |
dc.description.sponsorship | National Institute of Biomedical Imaging and Bioengineering (U.S.) | en_US |
dc.description.sponsorship | Howard Hughes Medical Institute | en_US |
dc.language.iso | en_US | |
dc.publisher | Society for Neuroscience | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1523/jneurosci.5968-10.2011 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | SFN | en_US |
dc.title | PSD-95 Is Required to Sustain the Molecular Organization of the Postsynaptic Density | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Chen, X. et al. “PSD-95 Is Required to Sustain the Molecular Organization of the Postsynaptic Density.” Journal of Neuroscience 31 (2011): 6329-6338. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences | en_US |
dc.contributor.department | Picower Institute for Learning and Memory | en_US |
dc.contributor.approver | Sheng, Morgan Hwa-Tze | |
dc.contributor.mitauthor | Nelson, Christopher D. | |
dc.contributor.mitauthor | Sheng, Morgan Hwa-Tze | |
dc.relation.journal | Journal of Neuroscience | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Chen, X.; Nelson, C. D.; Li, X.; Winters, C. A.; Azzam, R.; Sousa, A. A.; Leapman, R. D.; Gainer, H.; Sheng, M.; Reese, T. S. | en |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |