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dc.contributor.authorReddien, Peter
dc.contributor.authorLapan, Sylvain W.
dc.date.accessioned2012-02-10T19:21:09Z
dc.date.available2012-02-10T19:21:09Z
dc.date.issued2011-08
dc.date.submitted2011-03
dc.identifier.issn1553-7390
dc.identifier.issn1553-7404
dc.identifier.urihttp://hdl.handle.net/1721.1/69087
dc.description.abstractOptic cups are a structural feature of diverse eyes, from simple pit eyes to camera eyes of vertebrates and cephalopods. We used the planarian prototypic eye as a model to study the genetic control of optic cup formation and regeneration. We identified two genes encoding transcription factors, sp6-9 and dlx, that were expressed in the eye specifically in the optic cup and not the photoreceptor neurons. RNAi of these genes prevented formation of visible optic cups during regeneration. Planarian regeneration requires an adult proliferative cell population with stem cell-like properties called the neoblasts. We found that optic cup formation occurred only after migration of progressively differentiating progenitor cells from the neoblast population. The eye regeneration defect caused by dlx and sp6-9 RNAi can be explained by a failure to generate these early optic cup progenitors. Dlx and Sp6-9 genes function as a module during the development of diverse animal appendages, including vertebrate and insect limbs. Our work reveals a novel function for this gene pair in the development of a fundamental eye component, and it utilizes these genes to demonstrate a mechanism for total organ regeneration in which extensive cell movement separates new cell specification from organ morphogenesis.en_US
dc.description.sponsorshipUnited States. National Institutes of Health (NIH R01GM080639)en_US
dc.description.sponsorshipW. M. Keck Foundationen_US
dc.description.sponsorshipHoward Hughes Medical Instituteen_US
dc.language.isoen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pgen.1002226en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/en_US
dc.sourcePLoSen_US
dc.titledlx and sp6-9 Control Optic Cup Regeneration in a Prototypic Eyeen_US
dc.typeArticleen_US
dc.identifier.citationLapan, Sylvain W., and Peter W. Reddien. “Dlx and Sp6-9 Control Optic Cup Regeneration in a Prototypic Eye.” Ed. Claude Desplan. PLoS Genetics 7.8 (2011): e1002226. Web. 10 Feb. 2012.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.approverReddien, Peter W.
dc.contributor.mitauthorLapan, Sylvain William
dc.contributor.mitauthorReddien, Peter
dc.relation.journalPLoS Geneticsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsLapan, Sylvain W.; Reddien, Peter W.en
dc.identifier.orcidhttps://orcid.org/0000-0002-5569-333X
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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