Diagnosing breast cancer using diffuse reflectance spectroscopy and intrinsic fluorescence spectroscopy
Name
Volynskaya-2008-Diagnosing breast ca.pdf
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Author(s)
Fitzmaurice, Maryann
Shenk, Robert
Wang, Nancy
Volynskaya, Zoya I.
Haka, Abigail S.
Bechtel, Kate L.
Nazemi, Jonathan H.
Dasari, Ramachandra Rao
Feld, Michael S., 1940-
Date Issued
April 2008
Journal
Journal of Biomedical Optics
Publisher
SPIE - International Society for Optical Engineering
Citation
Volynskaya, Zoya et al. “Diagnosing Breast Cancer Using Diffuse Reflectance Spectroscopy and Intrinsic Fluorescence Spectroscopy.” Journal of Biomedical Optics 13.2 (2008): 024012. Web. 16 Feb. 2012. © 2008 SPIE - International Society for Optical Engineering
Version
Final published version
Abstract
Using diffuse reflectance spectroscopy and intrinsic fluorescence spectroscopy, we have developed an algorithm that successfully classifies normal breast tissue, fibrocystic change, fibroadenoma, and infiltrating ductal carcinoma in terms of physically meaningful parameters. We acquire 202 spectra from 104 sites in freshly excised breast biopsies from 17 patients within 30 min of surgical excision. The broadband diffuse reflectance and fluorescence spectra are collected via a portable clinical spectrometer and specially designed optical fiber probe. The diffuse reflectance spectra are fit using modified diffusion theory to extract absorption and scattering tissue parameters. Intrinsic fluorescence spectra are extracted from the combined fluorescence and diffuse reflectance spectra and analyzed using multivariate curve resolution. Spectroscopy results are compared to pathology diagnoses, and diagnostic algorithms are developed based on parameters obtained via logistic regression with cross-validation. The sensitivity, specificity, positive predictive value, negative predictive value, and overall diagnostic accuracy (total efficiency) of the algorithm are 100, 96, 69, 100, and 91%, respectively. All invasive breast cancer specimens are correctly diagnosed. The combination of diffuse reflectance spectroscopy and intrinsic fluorescence spectroscopy yields promising results for discrimination of breast cancer from benign breast lesions and warrants a prospective clinical study.
MIT Department
Massachusetts Institute of Technology. Laser Biomedical Research Center
Massachusetts Institute of Technology. Spectroscopy Laboratory
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DOI of Published Version
https://doi.org/10.1117/1.2909672
