Show simple item record

dc.contributor.authorChurch, Matthew J.
dc.contributor.authorWai, Brenner
dc.contributor.authorKarl, David M.
dc.contributor.authorDeLong, Edward
dc.date.accessioned2012-02-28T15:47:08Z
dc.date.available2012-02-28T15:47:08Z
dc.date.issued2010-03
dc.date.submitted2009-07
dc.identifier.issn1462-2920
dc.identifier.urihttp://hdl.handle.net/1721.1/69223
dc.description.abstractPlanktonic Crenarchaea are thought to play a key role in chemolithotrophic ammonia oxidation, a critical step of the marine nitrogen (N) cycle. In this study, we examined the spatial distributions of ammonia-oxidizing Crenarchaea across a large (∼5200 km) region of the central Pacific Ocean. Examination of crenarchaeal 16S rRNA, ammonia monooxygenase subunit A (amoA) genes, and amoA transcript abundances provided insight into their spatial distributions and activities. Crenarchaeal gene abundances increased three to four orders of magnitude with depth between the upper ocean waters and dimly lit waters of the mesopelagic zone. The resulting median value of the crenarchaeal amoA: 16S rRNA gene ratio was 1.3, suggesting the majority of Crenarchaea in the epi- and mesopelagic regions of the Pacific Ocean have the metabolic machinery for ammonia oxidation. Crenarchaeal amoA transcript abundances typically increased one to two orders of magnitude in the transitional zone separating the epipelagic waters from the mesopelagic (100–200 m), before decreasing into the interior of the mesopelagic zone. The resulting gene copy normalized transcript abundances revealed elevated amoA expression in the upper ocean waters (0–100 m) where crenarchaeal abundances were low, with transcripts decreasing into the mesopelagic zone as crenarchaeal gene abundances increased. These results suggest ammonia-oxidizing Crenarchaea are active contributors to the N cycle throughout the epi- and mesopelagic waters of the Pacific Ocean.en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (OCE 0425363)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (OCE03-26616)en_US
dc.description.sponsorshipUniversity of Hawaii. C-MORE Scholars Program (EF 0424599)en_US
dc.description.sponsorshipGordon and Betty Moore Foundation (Marine Microbiology Initiative)en_US
dc.language.isoen_US
dc.publisherJohn Wiley & Sons, Inc.en_US
dc.relation.isversionofhttp://dx.doi.org/10.1111/j.1462-2920.2009.02108.xen_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourceDeLong via Anne Grahamen_US
dc.titleDistributions of crenarchaeal amoA genes and transcripts in the Pacific Oceanen_US
dc.title.alternativeAbundances of crenarchaeal amoA genes and transcripts in the Pacific Oceanen_US
dc.typeArticleen_US
dc.identifier.citationChurch, Matthew J. et al. “Abundances of Crenarchaeal amoA Genes and Transcripts in the Pacific Ocean.” Environmental Microbiology 12.3 (2010): 679–688.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Civil and Environmental Engineeringen_US
dc.contributor.approverDeLong, Edward
dc.contributor.mitauthorDeLong, Edward
dc.relation.journalEnvironmental Microbiologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsChurch, Matthew J.; Wai, Brenner; Karl, David M.; DeLong, Edward F.en
mit.licenseOPEN_ACCESS_POLICYen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record