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dc.contributor.authorYassour, Moran
dc.contributor.authorPfiffner, Jenna
dc.contributor.authorLevin, Joshua Z.
dc.contributor.authorAdiconis, Xian
dc.contributor.authorGnirke, Andreas
dc.contributor.authorNusbaum, Chad
dc.contributor.authorThompson, Dawn-Anne
dc.contributor.authorFriedman, Nir
dc.contributor.authorRegev, Aviv
dc.contributor.authorYassour, Moran
dc.date.accessioned2012-03-09T20:05:41Z
dc.date.available2012-03-09T20:05:41Z
dc.date.issued2010-08
dc.date.submitted2010-07
dc.identifier.issn1465-6906
dc.identifier.issn1474-7596
dc.identifier.urihttp://hdl.handle.net/1721.1/69638
dc.description.abstractBackground Recent studies in budding yeast have shown that antisense transcription occurs at many loci. However, the functional role of antisense transcripts has been demonstrated only in a few cases and it has been suggested that most antisense transcripts may result from promiscuous bi-directional transcription in a dense genome. Results Here, we use strand-specific RNA sequencing to study anti-sense transcription in Saccharomyces cerevisiae. We detect 1,103 putative antisense transcripts expressed in mid-log phase growth, ranging from 39 short transcripts covering only the 3' UTR of sense genes to 145 long transcripts covering the entire sense open reading frame. Many of these antisense transcripts overlap sense genes that are repressed in mid-log phase and are important in stationary phase, stress response, or meiosis. We validate the differential regulation of 67 antisense transcripts and their sense targets in relevant conditions, including nutrient limitation and environmental stresses. Moreover, we show that several antisense transcripts and, in some cases, their differential expression have been conserved across five species of yeast spanning 150 million years of evolution. Divergence in the regulation of antisense transcripts to two respiratory genes coincides with the evolution of respiro-fermentation. Conclusions Our work provides support for a global and conserved role for antisense transcription in yeast gene regulation.en_US
dc.description.sponsorshipCanadian Friends of the Hebrew Universityen_US
dc.description.sponsorshipHoward Hughes Medical Instituteen_US
dc.description.sponsorshipHuman Frontier Science Program (Strasbourg, France)en_US
dc.description.sponsorshipBurroughs Wellcome Fund (Career Award at the Scientific Interface)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.). Pioneer Awarden_US
dc.description.sponsorshipBroad Institute of MIT and Harvarden_US
dc.description.sponsorshipU.S.-Israel Binational Science Foundation (BSF)en_US
dc.description.sponsorshipNational Human Genome Research Institute (U.S.)en_US
dc.description.sponsorshipAlfred P. Sloan Foundationen_US
dc.language.isoen_US
dc.publisherSpringer (Biomed Central Ltd.)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1186/gb-2010-11-8-r87en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_US
dc.sourceBioMed Centralen_US
dc.titleStrand-specific RNA sequencing reveals extensive regulated long antisense transcripts that are conserved across yeast speciesen_US
dc.typeArticleen_US
dc.identifier.citationYassour, Moran et al. “Strand-specific RNA Sequencing Reveals Extensive Regulated Long Antisense Transcripts That Are Conserved Across Yeast Species.” Genome Biology 11.8 (2010): R87. Web. 9 Mar. 2012.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.approverRegev, Aviv
dc.contributor.mitauthorFriedman, Nir
dc.contributor.mitauthorRegev, Aviv
dc.contributor.mitauthorYassour, Moran
dc.relation.journalGenome Biologyen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsYassour, Moran; Pfiffner, Jenna; Levin, Joshua Z; Adiconis, Xian; Gnirke, Andreas; Nusbaum, Chad; Thompson, Dawn-Anne; Friedman, Nir; Regev, Aviven
dc.identifier.orcidhttps://orcid.org/0000-0001-8567-2049
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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