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Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.

Author(s)
Love, Kerry Routenberg; Panagiotou, Vasiliki; Jiang, Bo; Stadheim, Terrance A.; Love, J. Christopher
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Abstract
The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion of a human Fc fragment by P. pastoris is not cell-cycle dependent, but rather fluctuates between states of high and low productivity in a stochastic manner.
Date issued
2010-06
URI
http://hdl.handle.net/1721.1/69920
Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Journal
Biotechnology and Bioengineering
Publisher
John Wiley & Sons, Inc.
Citation
Love, Kerry Routenberg et al. “Integrated Single-cell Analysis Shows Pichia Pastoris Secretes Protein Stochastically.” Biotechnology and Bioengineering, 106.2 (2010): 319–325.
Version: Author's final manuscript
ISSN
0006-3592
1097-0290

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