Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.
Author(s)Love, Kerry Routenberg; Panagiotou, Vasiliki; Jiang, Bo; Stadheim, Terrance A.; Love, J. Christopher
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The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion of a human Fc fragment by P. pastoris is not cell-cycle dependent, but rather fluctuates between states of high and low productivity in a stochastic manner.
DepartmentMassachusetts Institute of Technology. Department of Chemical Engineering
Biotechnology and Bioengineering
John Wiley & Sons, Inc.
Love, Kerry Routenberg et al. “Integrated Single-cell Analysis Shows Pichia Pastoris Secretes Protein Stochastically.” Biotechnology and Bioengineering, 106.2 (2010): 319–325.
Author's final manuscript