Steering trajectories of rolling cells by 2D asymmetric receptor patterning

Abstract

We demonstrate a simple method to make high-resolution P-selectin patterns based on microcontact printing for controlling cell rolling. SEM characterization revealed well-defined patterns that could direct the trajectories of rolling HL60 cells along the edges. Cell tracking revealed that velocities of rolling cells were higher at the edges as compared to plain P-selectin regions, with a maximum at an edge angle of 20°. The distance traveled by the HL60 cells along the edges decreased with increasing edge angles. These substrates may be used to tune cell adhesion and control the transport of the cells for separation and analysis.