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dc.contributor.authorKimura, Hiroyuki
dc.contributor.authorYoung, Curtis Robert, III
dc.contributor.authorMartinez, Asuncion
dc.contributor.authorDeLong, Edward
dc.date.accessioned2012-04-12T19:56:17Z
dc.date.available2012-04-12T19:56:17Z
dc.date.issued2011-04
dc.date.submitted2011-02
dc.identifier.issn1751-7362
dc.identifier.issn1751-7370
dc.identifier.urihttp://hdl.handle.net/1721.1/70007
dc.description.abstractProteorhodopsin (PR) is a photoprotein that functions as a light-driven proton pump in diverse marine Bacteria and Archaea. Recent studies have suggested that PR may enhance both growth rate and yield in some flavobacteria when grown under nutrient-limiting conditions in the light. The direct involvement of PR, and the metabolic details enabling light-stimulated growth, however, remain uncertain. Here, we surveyed transcriptional and growth responses of a PR-containing marine flavobacterium during carbon-limited growth in the light and the dark. As previously reported (Gómez-Consarnau et al., 2007), Dokdonia strain MED134 exhibited light-enhanced growth rates and cell yields under low carbon growth conditions. Inhibition of retinal biosynthesis abolished the light-stimulated growth response, supporting a direct role for retinal-bound PR in light-enhanced growth. Among protein-coding transcripts, both PR and retinal biosynthetic enzymes showed significant upregulation in the light. Other light-associated proteins, including bacterial cryptochrome and DNA photolyase, were also expressed at significantly higher levels in the light. Membrane transporters for Na+/phosphate and Na+/alanine symporters, and the Na+-translocating NADH-quinone oxidoreductase (NQR) linked electron transport chain, were also significantly upregulated in the light. Culture experiments using a specific inhibitor of Na+-translocating NQR indicated that sodium pumping via NQR is a critical metabolic process in the light-stimulated growth of MED134. In total, the results suggested the importance of both the PR-enabled, light-driven proton gradient, as well as the generation of a Na+ ion gradient, as essential components for light-enhanced growth in these flavobacteria.en_US
dc.description.sponsorshipGordon and Betty Moore Foundationen_US
dc.description.sponsorshipNational Science Foundation (U.S.) (NSF Science and Technology Center Award EF0424599.)en_US
dc.description.sponsorshipJapan Society for the Promotion of Science (Postdoctoral Fellowships for Research Abroad)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/ismej.2011.36en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourceDeLong via Anne Grahamen_US
dc.titleLight-induced transcriptional responses associated with proteorhodopsin-enhanced growth in a marine flavobacteriumen_US
dc.typeArticleen_US
dc.identifier.citationKimura, Hiroyuki et al. “Light-induced Transcriptional Responses Associated with Proteorhodopsin-enhanced Growth in a Marine Flavobacterium.” The ISME Journal 5.10 (2011): 1641–1651. Web.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Civil and Environmental Engineeringen_US
dc.contributor.approverDeLong, Edward
dc.contributor.mitauthorMartinez, Asuncion
dc.contributor.mitauthorYoung, Curtis Robert, III
dc.contributor.mitauthorKimura, Hiroyuki
dc.contributor.mitauthorDeLong, Edward
dc.relation.journalISME Journalen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsKimura, Hiroyuki; Young, Curtis R; Martinez, Asuncion; DeLong, Edward Fen
mit.licenseOPEN_ACCESS_POLICYen_US


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