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Validating Transcripts with Probes and Imaging Technology

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dc.contributor.author Itzkovitz, Shaul Shalev
dc.contributor.author van Oudenaarden, Alexander
dc.date.accessioned 2012-06-04T17:24:57Z
dc.date.available 2012-06-04T17:24:57Z
dc.date.issued 2011-03
dc.identifier.issn 1548-7091
dc.identifier.issn 1548-7105
dc.identifier.uri http://hdl.handle.net/1721.1/71013
dc.description.abstract High-throughput gene expression screens provide a quantitative picture of the average expression signature of biological samples. However, the analysis of spatial gene expression patterns with single-cell resolution requires quantitative in situ measurement techniques. Here we describe recent technological advances in RNA fluorescence in situ hybridization (FISH) techniques that facilitate detection of individual fluorescently labeled mRNA molecules of practically any endogenous gene. These methods, which are based on advances in probe design, imaging technology and image processing, enable the absolute measurement of transcript abundance in individual cells with single-molecule resolution. en_US
dc.description.sponsorship National Cancer Institute (U.S.). Physical Sciences-Oncology Center (U54CA143874) en_US
dc.description.sponsorship National Institutes of Health (U.S.) (Pioneer award 1DP1OD003936) en_US
dc.language.iso en_US
dc.publisher Nature Publishing Group en_US
dc.relation.isversionof http://dx.doi.org/10.1038/nmeth.1573 en_US
dc.rights Creative Commons Attribution-Noncommercial-Share Alike 3.0 en_US
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/ en_US
dc.source Prof. van Oudenaarden via Mat Wilmott en_US
dc.title Validating Transcripts with Probes and Imaging Technology en_US
dc.type Article en_US
dc.identifier.citation Itzkovitz, Shalev, and Alexander van Oudenaarden. “Validating transcripts with probes and imaging technology.” Nature Methods 8.4s (2011): S12-S19. en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Physics en_US
dc.contributor.department Massachusetts Institute of Technology. Materials Processing Center en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Biology en_US
dc.contributor.department David H. Koch Institute for Integrative Cancer Research at MIT en_US
dc.contributor.approver van Oudenaarden, Alexander
dc.contributor.mitauthor Itzkovitz, Shaul Shalev
dc.contributor.mitauthor van Oudenaarden, Alexander
dc.relation.journal Nature Methods en_US
dc.identifier.mitlicense OPEN_ACCESS_POLICY en_US
dc.eprint.version Author's final manuscript en_US
dc.type.uri http://purl.org/eprint/type/JournalArticle en_US
eprint.status http://purl.org/eprint/status/PeerReviewed en_US
dspace.orderedauthors Itzkovitz, Shalev; van Oudenaarden, Alexander en


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