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dc.contributor.authorCalderon de Anda, Froylan
dc.contributor.authorMeletis, Konstantinos
dc.contributor.authorGe, Xuecai
dc.contributor.authorRei, Damien
dc.contributor.authorTsai, Li-Huei
dc.date.accessioned2012-06-27T16:59:32Z
dc.date.available2012-06-27T16:59:32Z
dc.date.issued2010-08
dc.identifier.issn0270-6474
dc.identifier.issn1529-2401
dc.identifier.urihttp://hdl.handle.net/1721.1/71218
dc.description.abstractThe mechanisms underlying the normal development of neuronal morphology remain a fundamental question in neurobiology. Studies in cultured neurons have suggested that the position of the centrosome and the Golgi may predict the site of axon outgrowth. During neuronal migration in the developing cortex, however, the centrosome and Golgi are oriented toward the cortical plate at a time when axons grow toward the ventricular zone. In the current work, we use in situ live imaging to demonstrate that the centrosome and the accompanying polarized cytoplasm exhibit apical translocation in newborn cortical neurons preceding initial axon outgrowth. Disruption of centrosomal activity or downregulation of the centriolar satellite protein PCM-1 affects axon formation. We further show that downregulation of the centrosomal protein Cep120 impairs microtubule organization, resulting in increased centrosome motility. Decreased centrosome motility resulting from microtubule stabilization causes an aberrant centrosomal localization, leading to misplaced axonal outgrowth. Our results reveal the dynamic nature of the centrosome in developing cortical neurons, and implicate centrosome translocation and microtubule organization during the multipolar stage as important determinants of axon formation.en_US
dc.description.sponsorshipSimons Initiative on Autismen_US
dc.description.sponsorshipThe Brain Infrastructure Grant Programen_US
dc.description.sponsorshipKnut and Alice Wallenberg Foundationen_US
dc.description.sponsorshipHoward Hughes Medical Instituteen_US
dc.language.isoen_US
dc.publisherSociety for Neuroscienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1523/jneurosci.0381-10.2010en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceSFNen_US
dc.titleCentrosome Motility Is Essential for Initial Axon Formation in the Neocortexen_US
dc.typeArticleen_US
dc.identifier.citationde Anda, F. C. et al. “Centrosome Motility Is Essential for Initial Axon Formation in the Neocortex.” Journal of Neuroscience 30.31 (2010): 10391–10406. Web. 27 June 2012.en_US
dc.contributor.departmentmove to dc.description.sponsorshipen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentPicower Institute for Learning and Memoryen_US
dc.contributor.approverTsai, Li-Huei
dc.contributor.mitauthorCalderon de Anda, Froylan
dc.contributor.mitauthorMeletis, Konstantinos
dc.contributor.mitauthorGe, Xuecai
dc.contributor.mitauthorRei, Damien
dc.contributor.mitauthorTsai, Li-Huei
dc.relation.journalJournal of Neuroscienceen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsde Anda, F. C.; Meletis, K.; Ge, X.; Rei, D.; Tsai, L.-H.en
dc.identifier.orcidhttps://orcid.org/0000-0003-1262-0592
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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