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Three-dimensional differential interference contrast microscopy using synthetic aperture imaging

Author(s)
Dasari, Ramachandra Rao; Feld, Michael S.; Sung, Yongjin; Kim, Moonseok; Choi, Youngwoon; Fang-Yen, Chris; Kim, Kwanhyung; Choi, Wonshik; ... Show more Show less
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Abstract
We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.
Date issued
2012-02
URI
http://hdl.handle.net/1721.1/71243
Department
Massachusetts Institute of Technology. Spectroscopy Laboratory
Journal
Journal of Biomedical Optics
Publisher
SPIE
Citation
Kim, Moonseok et al. “Three-dimensional Differential Interference Contrast Microscopy Using Synthetic Aperture Imaging.” Journal of Biomedical Optics 17.2 (2012): 026003. © 2012 SPIE
Version: Final published version
ISSN
1083-3668
1560-2281

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