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ATP-Sensitive Potassium Channels Exhibit Variance in the Number of Open Channels below the Limit Predicted for Identical and Independent Gating

Author(s)
Choi, Kee-Hyun; Licht, Stuart
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Abstract
In small cells containing small numbers of ion channels, noise due to stochastic channel opening and closing can introduce a substantial level of variability into the cell's membrane potential. Negatively cooperative interactions that couple a channel's gating conformational change to the conformation of its neighbor(s) provide a potential mechanism for mitigating this variability, but such interactions have not previously been directly observed. Here we show that heterologously expressed ATP-sensitive potassium channels generate noise (i.e., variance in the number of open channels) below the level possible for identical and independent channels. Kinetic analysis with single-molecule resolution supports the interpretation that interchannel negative cooperativity (specifically, the presence of an open channel making a closed channel less likely to open) contributes to the decrease in noise. Functional coupling between channels may be important in modulating stochastic fluctuations in cellular signaling pathways.
Date issued
2012-05
URI
http://hdl.handle.net/1721.1/71759
Department
Massachusetts Institute of Technology. Department of Chemistry
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Choi, Kee-Hyun, and Stuart Licht. “ATP-Sensitive Potassium Channels Exhibit Variance in the Number of Open Channels Below the Limit Predicted for Identical and Independent Gating.” Ed. Floyd Romesberg. PLoS ONE 7.5 (2012): e37399.
Version: Final published version

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