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dc.contributor.authorBokinsky, Gregory
dc.contributor.authorPeralta-Yahya, Pamela P.
dc.contributor.authorGeorge, Anthe
dc.contributor.authorHolmes, Bradley M.
dc.contributor.authorSteen, Eric J.
dc.contributor.authorDietrich, Jeffrey
dc.contributor.authorLee, Taek Soon
dc.contributor.authorTullman-Ercek, Danielle
dc.contributor.authorVoigt, Christopher A.
dc.contributor.authorSimmons, Blake A.
dc.contributor.authorKeasling, Jay D.
dc.date.accessioned2012-07-26T18:44:15Z
dc.date.available2012-07-26T18:44:15Z
dc.date.issued2011-11
dc.date.submitted2011-05
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.urihttp://hdl.handle.net/1721.1/71847
dc.description.abstractOne approach to reducing the costs of advanced biofuel production from cellulosic biomass is to engineer a single microorganism to both digest plant biomass and produce hydrocarbons that have the properties of petrochemical fuels. Such an organism would require pathways for hydrocarbon production and the capacity to secrete sufficient enzymes to efficiently hydrolyze cellulose and hemicellulose. To demonstrate how one might engineer and coordinate all of the necessary components for a biomass-degrading, hydrocarbon-producing microorganism, we engineered a microorganism naïve to both processes, Escherichia coli, to grow using both the cellulose and hemicellulose fractions of several types of plant biomass pretreated with ionic liquids. Our engineered strains express cellulase, xylanase, beta-glucosidase, and xylobiosidase enzymes under control of native E. coli promoters selected to optimize growth on model cellulosic and hemicellulosic substrates. Furthermore, our strains grow using either the cellulose or hemicellulose components of ionic liquid-pretreated biomass or on both components when combined as a coculture. Both cellulolytic and hemicellulolytic strains were further engineered with three biofuel synthesis pathways to demonstrate the production of fuel substitutes or precursors suitable for gasoline, diesel, and jet engines directly from ionic liquid-treated switchgrass without externally supplied hydrolase enzymes. This demonstration represents a major advance toward realizing a consolidated bioprocess. With improvements in both biofuel synthesis pathways and biomass digestion capabilities, our approach could provide an economical route to production of advanced biofuels.en_US
dc.description.sponsorshipUnited States. Dept. of Energy. Office of Biological and Environmental Research (contract DE-AC02-05CH11231)en_US
dc.description.sponsorshipNational Science Foundation (U.S.). (Grant BES-0547637)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant AI67699)en_US
dc.description.sponsorshipUniversity of California Discovery Grant (bio05-10556)en_US
dc.language.isoen_US
dc.publisherNational Academy of Sciences of the United States of Americaen_US
dc.relation.isversionofhttp://dx.doi.org/10.1073/pnas.1106958108en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePNASen_US
dc.titleSynthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia colien_US
dc.typeArticleen_US
dc.identifier.citationBokinsky, G. et al. “Synthesis of Three Advanced Biofuels from Ionic Liquid-pretreated Switchgrass Using Engineered Escherichia Coli.” Proceedings of the National Academy of Sciences 108.50 (2011): 19949–19954. Copyright ©2011 by the National Academy of Sciencesen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.approverVoigt, Christopher A.
dc.contributor.mitauthorVoigt, Christopher A.
dc.relation.journalProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsBokinsky, G.; Peralta-Yahya, P. P.; George, A.; Holmes, B. M.; Steen, E. J.; Dietrich, J.; Soon Lee, T.; Tullman-Ercek, D.; Voigt, C. A.; Simmons, B. A.; Keasling, J. D.en
dc.identifier.orcidhttps://orcid.org/0000-0003-0844-4776
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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