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Structural Characterization of GNNQQNY Amyloid Fibrils by Magic Angle Spinning NMR

Author(s)
Griffin, Robert Guy; Lewandowski, Jozef R.; van der Wel, Patrick C. A.
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Abstract
Several human diseases are associated with the formation of amyloid aggregates, but experimental characterization of these amyloid fibrils and their oligomeric precursors has remained challenging. Experimental and computational analysis of simpler model systems has therefore been necessary, for instance, on the peptide fragment GNNQQNY[subscript 7−13] of yeast prion protein Sup35p. Expanding on a previous publication, we report here a detailed structural characterization of GNNQQNY fibrils using magic angle spinning (MAS) NMR. On the basis of additional chemical shift assignments we confirm the coexistence of three distinct peptide conformations within the fibrillar samples, as reflected in substantial chemical shift differences. Backbone torsion angle measurements indicate that the basic structure of these coexisting conformers is an extended β-sheet. We structurally characterize a previously identified localized distortion of the β-strand backbone specific to one of the conformers. Intermolecular contacts are consistent with each of the conformers being present in its own parallel and in-register sheet. Overall the MAS NMR data indicate a substantial difference between the structure of the fibrillar and crystalline forms of these peptides, with a clearly increased complexity in the GNNQQNY fibril structure. These experimental data can provide guidance for future work, both experimental and theoretical, and provide insights into the distinction between fibril growth and crystal formation.
Date issued
2010-08
URI
http://hdl.handle.net/1721.1/72020
Department
Massachusetts Institute of Technology. Department of Chemistry; Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology)
Journal
Biochemistry
Publisher
American Chemical Society (ACS)
Citation
van der Wel, Patrick C. A., Józef R. Lewandowski, and Robert G. Griffin. “Structural Characterization of GNNQQNY Amyloid Fibrils by Magic Angle Spinning NMR.” Biochemistry 49.44 (2010): 9457–9469.
Version: Author's final manuscript
ISSN
0006-2960
1520-4995

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