dc.contributor.author | Nam, Jin-Wu | |
dc.contributor.author | Bartel, David | |
dc.date.accessioned | 2012-08-30T14:37:02Z | |
dc.date.available | 2012-08-30T14:37:02Z | |
dc.date.issued | 2012-06 | |
dc.date.submitted | 2012-03 | |
dc.identifier.issn | 1088-9051 | |
dc.identifier.issn | 1088-9051 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/72451 | |
dc.description.abstract | Thousands of long non-coding RNAs (lncRNAs) have been found in vertebrate animals, a few of which have known biological roles. To better understand the genomics and features of lncRNAs in invertebrates, we used available RNA-seq, poly(A)-site, and ribosome-mapping data to identify lncRNAs of C. elegans. We found 170 long intervening ncRNAs (lincRNAs), which had single- or multi-exonic structures that did not overlap protein-coding transcripts, and about sixty antisense lncRNAs (ancRNAs), which were complementary to protein-coding transcripts. Compared to protein-coding genes, the lncRNA genes tended to be expressed in stage-dependent manner. Approximately 25% of the newly identified lincRNAs showed little signal for sequence conservation and mapped antisense to clusters of endogenous siRNAs, as would be expected if they serve as templates and targets for these siRNAs. The other 75% tended to be more conserved and included lincRNAs with intriguing expression and sequence features associating them with processes such as dauer formation, male identity, sperm formation, and interaction with sperm-specific mRNAs. Our study provides a glimpse into the lncRNA content of a non-vertebrate animal and a resource for future studies of lncRNA function. | en_US |
dc.language.iso | en_US | |
dc.publisher | Cold Spring Harbor Laboratory Press | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1101/gr.140475.112 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike 3.0 | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | en_US |
dc.source | MIT web domain | en_US |
dc.title | Long non-coding RNAs in C. elegans | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Nam, J.-W., and D. Bartel. “Long Non-coding RNAs in C. Elegans.” Genome Research (2012). | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.approver | Bartel, David | |
dc.contributor.mitauthor | Nam, Jin-Wu | |
dc.contributor.mitauthor | Bartel, David | |
dc.relation.journal | Genome Research | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Nam, J.-W.; Bartel, D. | en |
dc.identifier.orcid | https://orcid.org/0000-0002-3872-2856 | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |