Quantitative cerebral blood flow with optical coherence tomography
Author(s)
Srinivasan, Vivek J.; Sakadžic, Sava; Gorczynska, Iwona; Ruvinskaya, Svetlana; Wu, Weicheng; Fujimoto, James G.; Boas, David A.; ... Show more Show less
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Absolute measurements of cerebral blood flow (CBF) are an important endpoint in studies of cerebral pathophysiology. Currently no accepted method exists for in vivo longitudinal monitoring of CBF with high resolution in rats and mice. Using three-dimensional Doppler Optical Coherence Tomography and cranial window preparations, we present methods and algorithms for regional CBF measurements in the rat cortex. Towards this end, we develop and validate a quantitative statistical model to describe the effect of static tissue on velocity sensitivity. This model is used to design scanning protocols and algorithms for sensitive 3D flow measurements and angiography of the cortex. We also introduce a method of absolute flow calculation that does not require explicit knowledge of vessel angles. We show that OCT estimates of absolute CBF values in rats agree with prior measures by autoradiography, suggesting that Doppler OCT can perform absolute flow measurements in animal models.
Date issued
2010-01Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Research Laboratory of ElectronicsJournal
Optics Express
Publisher
Optical Society of America
Citation
Srinivasan, Vivek J. et al. “Quantitative Cerebral Blood Flow with Optical Coherence Tomography.” Optics Express 18.3 (2010): 2477.
Version: Author's final manuscript
ISSN
1094-4087