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dc.contributor.authorRattanama, Pimonsri
dc.contributor.authorThompson, Janelle Renee
dc.contributor.authorKongkerd, Natthawan
dc.contributor.authorSrinitiwarawong, Kanchana
dc.contributor.authorVuddhakul, Varaporn
dc.contributor.authorMekalanos, John J.
dc.date.accessioned2012-09-14T14:54:29Z
dc.date.available2012-09-14T14:54:29Z
dc.date.issued2012-02
dc.date.submitted2011-07
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1721.1/72954
dc.description.abstractMembers of the genus Vibrio are important marine and aquaculture pathogens. Hemolytic activity has been identified as a virulence factor in many pathogenic vibrios including V. cholerae, V. parahaemolyticus, V. alginolyticus, V. harveyi and V. vulnificus. We have used transposon mutagenesis to identify genes involved in the hemolytic activity of shrimp-pathogenic V. harveyi strain PSU3316. Out of 1,764 mutants screened, five mutants showed reduced hemolytic activity on sheep blood agar and exhibited virulence attenuation in shrimp (Litopenaeus vannamei). Mutants were identified by comparing transposon junction sequences to a draft of assembly of the PSU3316 genome. Surprisingly none of the disrupted open reading frames or gene neighborhoods contained genes annotated as hemolysins. The gene encoding RseB, a negative regulator of the sigma factor (σE), was interrupted in 2 out of 5 transposon mutants, in addition, the transcription factor CytR, a threonine synthetase, and an efflux-associated cytoplasmic protein were also identified. Knockout mutations introduced into the rpoE operon at the rseB gene exhibited low hemolytic activity in sheep blood agar, and were 3-to 7-fold attenuated for colonization in shrimp. Comparison of whole cell extracted proteins in the rseB mutant (PSU4030) to the wild-type by 2-D gel electrophoresis revealed 6 differentially expressed proteins, including two down-regulated porins (OmpC-like and OmpN) and an upregulated protease (DegQ) which have been associated with σE in other organisms. Our study is the first report linking hemolytic activity to the σE regulators in pathogenic Vibrio species and suggests expression of this virulence-linked phenotype is governed by multiple regulatory pathways within the V. harveyi.en_US
dc.description.sponsorshipThailand Research Fund (Royal Golden Jubilee Ph.D. program (Grant No. HD/0153/2547))en_US
dc.description.sponsorshipHarvard Universityen_US
dc.description.sponsorshipMassachusetts Institute of Technologyen_US
dc.language.isoen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pone.0032523en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/en_US
dc.sourcePLoSen_US
dc.titleSigma E Regulators Control Hemolytic Activity and Virulence in a Shrimp Pathogenic Vibrio harveyien_US
dc.typeArticleen_US
dc.identifier.citationRattanama, Pimonsri et al. “Sigma E Regulators Control Hemolytic Activity and Virulence in a Shrimp Pathogenic Vibrio Harveyi.” Ed. Sarah K. Highlander. PLoS ONE 7.2 (2012): e32523. Web.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Civil and Environmental Engineeringen_US
dc.contributor.approverThompson, Janelle Renee
dc.contributor.mitauthorThompson, Janelle Renee
dc.relation.journalPLoS ONEen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsRattanama, Pimonsri; Thompson, Janelle R.; Kongkerd, Natthawan; Srinitiwarawong, Kanchana; Vuddhakul, Varaporn; Mekalanos, John J.en
mit.licensePUBLISHER_CCen_US


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