Design, construction and characterization of a set of insulated bacterial promoters
Author(s)Davis, Joseph Harry; Rubin, Adam J.; Sauer, Robert T.
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We have generated a series of variable-strength, constitutive, bacterial promoters that act predictably in different sequence contexts, span two orders of magnitude in strength and contain convenient sites for cloning and the introduction of downstream open-reading frames. Importantly, their design insulates these promoters from the stimulatory or repressive effects of many 5′- or 3′-sequence elements. We show that different promoters from our library produce constant relative levels of two different proteins in multiple genetic contexts. This set of promoters should be a useful resource for the synthetic-biology community.
DepartmentMassachusetts Institute of Technology. Department of Biology
Nucleic Acids Research
Oxford University Press
Davis, J. H., A. J. Rubin, and R. T. Sauer. “Design, Construction and Characterization of a Set of Insulated Bacterial Promoters.” Nucleic Acids Research 39.3 (2010): 1131–1141. Web.
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