Freely Suspended Cellular “Backpacks” Lead to Cell Aggregate Self-Assembly
Author(s)Irvine, Darrell J.; Cohen, Robert E.; Rubner, Michael F.; Gilbert, Jonathan Brian; Swiston, Albert J., Jr.
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Cellular “backpacks” are a new type of anisotropic, nanoscale thickness microparticle that may be attached to the surface of living cells creating a “bio-hybrid” material. Previous work has shown that these backpacks do not impair cell viability or native functions such as migration in a B and T cell line, respectively. In the current work, we show that backpacks, when added to a cell suspension, assemble cells into aggregates of reproducible size. We investigate the efficiency of backpack−cell binding using flow cytometry and laser diffraction, examine the influence of backpack diameter on aggregate size, and show that even when cell−backpack complexes are forced through small pores, backpacks are not removed from the surfaces of cells.
DepartmentMassachusetts Institute of Technology. Center for Materials Science and Engineering; Lincoln Laboratory; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Department of Materials Science and Engineering
American Chemical Society (ACS)
Swiston, Albert J. et al. “Freely Suspended Cellular ‘Backpacks’ Lead to Cell Aggregate Self-Assembly.” Biomacromolecules 11.7 (2010): 1826–1832. © 2010 American Chemical Society
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