Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis
Author(s)Barresi, Michael J. F.; Burton, Sean; Dipietrantonio, Kristina; Amsterdam, Adam; Hopkins, Nancy H.; Karlstrom, Rolf O.; ... Show more Show less
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The formation of the central nervous system depends on the coordinated development of neural and glial cell types that arise from a common precursor. Using an existing group of zebrafish mutants generated by viral insertion, we performed a “shelf-screen” to identify genes necessary for astroglial development and axon scaffold formation. We screened 274 of 315 viral insertion lines using antibodies that label axons (anti-Acetylated Tubulin) and astroglia (anti-Gfap) and identified 25 mutants with defects in gliogenesis, glial patterning, neurogenesis, and axon guidance. We also identified a novel class of mutants affecting radial glial cell numbers. Defects in astroglial patterning were always associated with axon defects, supporting an important role for axon-glial interactions during axon scaffold development. The genes disrupted in these viral lines have all been identified, providing a powerful new resource for the study of axon guidance, glio- and neurogenesis, and neuron-glial interactions during development of the vertebrate CNS.
DepartmentMassachusetts Institute of Technology. Department of Biology; Koch Institute for Integrative Cancer Research at MIT
Wiley Blackwell (John Wiley & Sons)
Barresi, Michael J.F. et al. “Essential Genes for Astroglial Development and Axon Pathfinding During Zebrafish Embryogenesis.” Developmental Dynamics 239.10 (2010): 2603–2618.
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