Direct and specific chemical control of eukaryotic translation with a synthetic RNA–protein interaction
Author(s)Goldfless, Stephen Jacob; Belmont, Brian Joshua; de Paz, Alexandra M.; Niles, Jacquin
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Sequence-specific RNA–protein interactions, though commonly used in biological systems to regulate translation, are challenging to selectively modulate. Here, we demonstrate the use of a chemically-inducible RNA–protein interaction to regulate eukaryotic translation. By genetically encoding Tet Repressor protein (TetR)-binding RNA elements into the 5′-untranslated region (5′-UTR) of an mRNA, translation of a downstream coding sequence is directly controlled by TetR and tetracycline analogs. In endogenous and synthetic 5′-UTR contexts, this system efficiently regulates the expression of multiple target genes, and is sufficiently stringent to distinguish functional from non-functional RNA–TetR interactions. Using a reverse TetR variant, we illustrate the potential for expanding the regulatory properties of the system through protein engineering strategies.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering
Nucleic Acids Research
Oxford University Press (OUP)
Goldfless, S. J. et al. “Direct and Specific Chemical Control of Eukaryotic Translation with a Synthetic RNA-protein Interaction.” Nucleic Acids Research 40.9 (2012): e64–e64.
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