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dc.contributor.authorHanna, Jacob
dc.contributor.authorMarkoulaki, Styliani
dc.contributor.authorMitalipova, Maisam
dc.contributor.authorCassady, John P.
dc.contributor.authorStaerk, Judith
dc.contributor.authorCarey, Bryce W.
dc.contributor.authorLengner, Christopher J.
dc.contributor.authorForeman, Ruth K.
dc.contributor.authorLove, Jennifer
dc.contributor.authorGao, Qing
dc.contributor.authorKim, Jongpil
dc.contributor.authorJaenisch, Rudolf
dc.contributor.authorCheng, Albert Wu
dc.date.accessioned2012-10-11T18:46:53Z
dc.date.available2012-10-11T18:46:53Z
dc.date.issued2009-05
dc.date.submitted2009-04
dc.identifier.issn1934-5909
dc.identifier.urihttp://hdl.handle.net/1721.1/73889
dc.description.abstractEmbryonic stem (ES) cells are isolated from the inner cell mass (ICM) of blastocysts, whereas epiblast stem cells (EpiSCs) are derived from the post-implantation epiblast and display a restricted developmental potential. Here we characterize pluripotent states in the non-obese diabetic (NOD) mouse strain, which prior to this study was considered “non-permissive” for ES cell derivation. We find that NOD stem cells can be stabilized by providing constitutive expression of Klf4 or c-Myc or small molecules that can replace these factors during in vitro reprogramming. The NOD ES and iPS cells appear “metastable”, as they acquire an alternative EpiSC-like identity after removal of the exogenous factors, while their reintroduction converts the cells back to ICM-like pluripotency. Our findings suggest that stem cells from different genetic backgrounds can assume distinct states of pluripotency in vitro, the stability of which is regulated by endogenous genetic determinants and can be modified by exogenous factors.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant RO1-HDO45022)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant R37-CA084198)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant RO1-CA087869)en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.stem.2009.04.015en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourcePMCen_US
dc.titleMetastable Pluripotent States in NOD Mouse Derived ES Cellsen_US
dc.typeArticleen_US
dc.identifier.citationHanna, Jacob et al. “Metastable Pluripotent States in NOD-Mouse-Derived ESCs.” Cell Stem Cell 4.6 (2009): 513–524.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Computational and Systems Biology Programen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorCheng, Albert W.
dc.contributor.mitauthorCassady, John P.
dc.contributor.mitauthorForeman, Ruth K.
dc.contributor.mitauthorJaenisch, Rudolf
dc.contributor.mitauthorCarey, Bryce W.
dc.relation.journalCell Stem Cellen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsHanna, Jacob; Markoulaki, Styliani; Mitalipova, Maisam; Cheng, Albert W.; Cassady, John P.; Staerk, Judith; Carey, Bryce W.; Lengner, Christopher J.; Foreman, Ruth; Love, Jennifer; Gao, Qing; Kim, Jongpil; Jaenisch, Rudolfen
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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