Seminaphthofluorescein-Based Fluorescent Probes for Imaging Nitric Oxide in Live Cells
Author(s)Pluth, Michael D.; Chan, Maria R.; McQuade, Lindsey E.; Lippard, Stephen J.
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Fluorescent turn-on probes for nitric oxide based on seminaphthofluorescein scaffolds were prepared and spectroscopically characterized. The Cu(II) complexes of these fluorescent probes react with NO under anaerobic conditions to yield a 20–45-fold increase in integrated emission. The seminaphthofluorescein-based probes emit at longer wavelengths than the parent FL1 and FL2 fluorescein-based generations of NO probes, maintaining emission maxima between 550 and 625 nm. The emission profiles depend on the excitation wavelength; maximum fluorescence turn-on is achieved at excitations between 535 and 575 nm. The probes are highly selective for NO over other biologically relevant reactive nitrogen and oxygen species including NO3–, NO2–, HNO, ONOO–, NO2, OCl–, and H2O2. The seminaphthofluorescein-based probes can be used to visualize endogenously produced NO in live cells, as demonstrated using Raw 264.7 macrophages.
DepartmentMassachusetts Institute of Technology. Department of Chemistry
American Chemical Society (ACS)
Pluth, Michael D. et al. “Seminaphthofluorescein-Based Fluorescent Probes for Imaging Nitric Oxide in Live Cells.” Inorganic Chemistry 50.19 (2011): 9385–9392.
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