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Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions

Author(s)
Saha, Krishanu; Mei, Ying; Reisterer, Colin M.; Pyzocha, Neena Kenton; Yang, Jing; Muffat, Julien; Davies, Martyn C.; Alexander, Morgan R.; Jaenisch, Rudolf; Langer, Robert S; Anderson, Daniel Griffith; ... Show more Show less
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Abstract
The current gold standard for the culture of human pluripotent stem cells requires the use of a feeder layer of cells. Here, we develop a spatially defined culture system based on UV/ozone radiation modification of typical cell culture plastics to define a favorable surface environment for human pluripotent stem cell culture. Chemical and geometrical optimization of the surfaces enables control of early cell aggregation from fully dissociated cells, as predicted from a numerical model of cell migration, and results in significant increases in cell growth of undifferentiated cells. These chemically defined xeno-free substrates generate more than three times the number of cells than feeder-containing substrates per surface area. Further, reprogramming and typical gene-targeting protocols can be readily performed on these engineered surfaces. These substrates provide an attractive cell culture platform for the production of clinically relevant factor-free reprogrammed cells from patient tissue samples and facilitate the definition of standardized scale-up friendly methods for disease modeling and cell therapeutic applications.
Date issued
2011-11
URI
http://hdl.handle.net/1721.1/74094
Department
Harvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemical Engineering; Koch Institute for Integrative Cancer Research at MIT
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Citation
Saha, K. et al. “Surface-engineered Substrates for Improved Human Pluripotent Stem Cell Culture Under Fully Defined Conditions.” Proceedings of the National Academy of Sciences 108.46 (2011): 18714–18719. Copyright ©2011 by the National Academy of Sciences
Version: Final published version
ISSN
0027-8424
1091-6490

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