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MiR-193b-365, a brown fat enriched microRNA cluster, is essential for brown fat differentiation

Author(s)
Sun, Lei; Xie, Huangming; Mori, Marcelo A.; Alexander, Ryan; Yuan, Bingbing B.; Hattangadi, Shilpa M.; Liu, Qingqing; Kahn, C. Ronald; Lodish, Harvey F; ... Show more Show less
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Alternative title
Mir193b-365 is essential for brown fat differentiation
Terms of use
Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/
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Abstract
Mammals have two principal types of fat. White adipose tissue primarily serves to store extra energy as triglycerides, whereas brown adipose tissue is specialized to burn lipids for heat generation and energy expenditure as a defence against cold and obesity1, 2. Recent studies have demonstrated that brown adipocytes arise in vivo from a Myf5-positive, myoblastic progenitor by the action of Prdm16 (PR domain containing 16). Here, we identified a brown-fat-enriched miRNA cluster, MiR-193b–365, as a key regulator of brown fat development. Blocking miR-193b and/or miR-365 in primary brown preadipocytes markedly impaired brown adipocyte adipogenesis by enhancing Runx1t1 (runt-related transcription factor 1; translocated to, 1) expression, whereas myogenic markers were significantly induced. Forced expression of Mir193b and/or Mir365 in C2C12 myoblasts blocked the entire programme of myogenesis, and, in adipogenic conditions, miR-193b induced myoblasts to differentiate into brown adipocytes. Mir193b–365 was upregulated by Prdm16 partially through Pparα. Our results demonstrate that Mir193b–365 serves as an essential regulator for brown fat differentiation, in part by repressing myogenesis.
Description
2012 February 1
Date issued
2011-07
URI
http://hdl.handle.net/1721.1/74180
Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry
Journal
Nature Cell Biology
Publisher
Nature Publishing Group
Citation
Sun, Lei et al. “Mir193b–365 Is Essential for Brown Fat Differentiation.” Nature Cell Biology 13.8 (2011): 958–965.
Version: Author's final manuscript
ISSN
1465-7392
1476-4679

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